二甲基鞘氨醇对人胃癌细胞凋亡的影响  

Effect of N,N' Dimethyl-sphingosine on human gastric carcinoma cell

作　　者：张锐梅 任娟[2] 张龙[2] 董蕾[3] 徐仓宝[4] 李宇[2] 李明众[2] 

机构地区：[1]深圳市龙岗区布吉人民医院,广东深圳518112 [2]西安交通大学第一医院肿瘤中心,陕西西安710061 [3]西安交通大学第二医院消化内科,陕西西安710004 [4]瑞典隆德大学,瑞典22100

出　　处：《现代肿瘤医学》2009年第7期1210-1212,共3页Journal of Modern Oncology

基　　金：西安交通大学博士基金资助项目(2001年)

摘　　要：目的:研究二甲基鞘氨醇对人胃癌细胞SGC7901凋亡的调节作用及其机制。方法:二甲基鞘氨醇是1-磷酸鞘氨醇生成的特异性抑制剂。应用二甲基鞘氨醇后,1-磷酸鞘氨醇的生成被抑制。流式细胞仪观察细胞DNA含量及细胞周期分布;用流式细胞仪检测细胞内Bcl-2和Bax的表达水平。结果:二甲基鞘氨醇抑制1-磷酸鞘氨醇生成后,①胃癌细胞显著发生凋亡。②胃癌细胞内Bcl-2/Bax表达水平的比值下降。结论:二甲基鞘氨醇在人胃癌细胞的凋亡调控中起重要作用。Objective：Sphingosine - 1 -phosphate （S1 P） is recently found an extraeellular signal transmitter and second intracellular messenger. The aim is to determine the role of S1P in regulating apoptosis of human gastric carcinoma cell and its mechanisms. Methods：Synthesis of S1 P can be blocked by N, N′ Dimethyl - sphingosine （DMS）.After the action of DMS, DNA content and cell cycle were determined by flow cytometry. Cellular protein expression level of Bcl -2 and Box was detected by their specific antibody through flow cytometry. Results ：①DMS induced apoptosis in SGC7901 ceils when reaching a certain dose. ②When the S1P synthesis was inhibited by DMS,the ratio of Bcl - 2 expression level to Bax expression level reduced. Conclusion ： Sphingosine - 1 - phosphate plays important roles in regulating the apoptosis of human gastric carcinoma cell SGC7901.

关 键 词：1-磷酸鞘氨醇 人胃癌细胞 二甲基鞘氨醇 

分 类 号：R730.23[医药卫生—肿瘤]