CXCR4基因RNA干扰慢病毒载体的构建与鉴定  

Construction and identification of lentiviral vector of RNA interference of CXCR4 gene

作　　者：高振军[1] 吴恺[1] 赵严[1] 胡国勇[1] 王兴鹏[1] 

机构地区：[1]上海交通大学附属第一人民医院消化科,上海市胰腺病重点实验室,上海200080

出　　处：《现代肿瘤医学》2009年第7期1215-1217,共3页Journal of Modern Oncology

基　　金：上海市胰腺病重点实验室专项基金(编号:05DZ22107)

摘　　要：目的:构建人CXCR4基因RNAi(RNA interference,RNAi)慢病毒载体。方法:针对筛选确定的人CXCR4基因RNAi有效靶序列,合成靶序列的OligoDNA,退火形成双链DNA,与经HpaI和XhoI酶切后的pGCL-GFP载体连接产生短发卡RNA慢病毒载体,PCR筛选阳性克隆,测序鉴定。结果:PCR鉴定与DNA测序证实合成的含CXCR4shRNA慢病毒载体寡核苷酸链插入正确。结论:成功构建人CXCR4基因RNAi慢病毒载体。Objective ：To construct a lentiviral vector of RNA interference （RNAi） of human CXCR4 gene. Methods ：The effective sequence of siRNA targeting CXCR4 gene was confirmed in our previous study. The complementary DNA containing both sense and antisense Oligo DNA of the targeting sequence was designed, synthesized and cloned into the pGCL- GFP vector,to construct a lentiviral vector which expressed short hairpin RNA （shRNA） ,and it was identified by PCR and DNA sequencing. Results：PCR identification and DNA sequencing demonstrated that insertion of oligonucleotide of the lentivirus RNAi vector containing human CXCR4 shRNA was right. Conclusion：The lentivirus RNAi vector of human CXCR4 was constructed successfully.

关 键 词：RNA干扰 CXCR4 慢病毒 

分 类 号：R73-362[医药卫生—肿瘤]