非小细胞肺癌(NSCLC)的LYVE-1阳性微淋巴管密度与肿瘤淋巴转移的关系  

LYVE-1 positive microlymphatic vessel density correlates with lymph metastasis in non-small cell lung cancer(NSCLC)

作　　者：汪源[1] 潘铁成[1] 陈涛[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院心胸外科,湖北武汉430030

出　　处：《现代肿瘤医学》2009年第7期1243-1245,共3页Journal of Modern Oncology

摘　　要：目的:评价LYVE-1作为肺癌淋巴管的特异性标志物的价值;探讨淋巴管生成与NSCLC淋巴转移的关系。方法:对40例NSCLC和8例组织石蜡切片进行免疫组化染色,检测LYVE-1、CD34的表达情况,并对LYVE-1和CD34阳性管腔密度进行计数。结果:LYVE-1阳性管腔密度(microlymphatic density,MLD)与CD34阳性管腔密度(microvascular density,MVD)无相关性(Spearman相关性分析,r=0.017,P>0.05),结合光镜下形态学特点,LYVE-1特异性表达于淋巴管内皮,而不在血管内皮表达。LYVE-1阳性MLD在NSCLC中显著高于良性肺组织,Ⅲ期+Ⅳ期显著高于Ⅰ期+Ⅱ期(P<0.05);与细胞分化程度无关(P>0.05)。有淋巴转移组LYVE-1阳性MLD显著高于无淋巴转移组(P<0.01),N2淋巴结转移组显著高于N1淋巴结转移组(P<0.05)。结论:LYVE-1对肺组织淋巴管内皮的表达的特异性较强,是研究NSCLC淋巴管增生较为理想的肿瘤淋巴管特异性标志物。NSCLC淋巴管增生对于肿瘤的发生、发展有重要作用,是促进肿瘤淋巴转移的重要因素。Objective： To evaluate the specificity of LYVE - 1 as a lymphatic endothelial marker. To investigate the relationship of lymphangiogenesis and the presence of lymph node metastasis. Methods：The expressions of CD34, LYVE - 1 were detected in 40 paraffin sections of NSCLC and 8 pulmonary benign disease tissues by immunohistochemistry （SP）. LYVE - 1 positive and CD34 positive vessel density were counted. Results：There was not a positive correlation between LYVE - 1 positive and CD34 positive vessel density （the Spearman rank correlation coefficient r =0. 017 ,P 〉0.05）, and none of LYVE- 1 positive vessels were blood vessels by observation of light microscope. LYVE - 1 positive MLD in NSCLC was significantly higher than that in pulmonary benign disease tissues （ P 〈 0.05 ）. Compared with the negative lymph node metastasis,positive lymph node metastasis group, LYVE- 1 positive MLD in the positive group increased significantly （P 〈0.01 ）. MLD was higher in stage Ⅲ and Ⅳ patients than that in stage Ⅰ and Ⅱ （ P 〈 0.01 ）. Conclusion： LYVE - 1 is a specific lymphatic endothelial marker. Lymphangiogenesis might play an important role in the lymph node metastasis of NSCLC.

关 键 词：LYVE-1 淋巴管密度 非小细胞肺癌 淋巴转移 淋巴管增生 

分 类 号：R734.2[医药卫生—肿瘤]