Comparative proteomic analysis of the response in resistant and susceptible maize inbred lines to infection by Curvularia lunata  

作　　者：Xiuli Huang 1, Lixing Liu 1, Jie Chen Yuhong Zhai Department of Plant Science, School of Agriculture and Biology, Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiaotong University, Shanghai 200240, China 

出　　处：《Progress in Natural Science:Materials International》2009年第7期845-850,共6页自然科学进展·国际材料（英文版）

基　　金：supported by the National Science &Technology Supporting Program of China (Grant No.2006BAD08A06);National Basic Research Program of China (Grant No. 2006CB101901);National Natural Science Foundation of China (Grant Nos. 30471057 and 30370917)

摘　　要：Proteins differentially expressed from maize leaves in response to the infection by Curvularia lunata strain CX-3 were identified through a high-resolution two-dimensional gel electrophoresis （2-DE） method. Two inbred lines, 78599-1 and E28, were used, respec- tively, as resistant and susceptible lines to CX-3 infection. Proteins were extracted from the fourth leaves of six-or seven-leaf stage plants sampled at 24, 36, 48, 60, and 72 h after inoculation with CX-3. Twenty-seven differentially expressed protein spots resolved on the 2-DE gels were identified by MALDI-TOF MS/MS. The results showed that these proteins are associated with photosynthesis, respiration, oxidative and drought stress tolerance as well as signal transduction in maize. Among stress-related proteins, the 22 kDa drought-inducible protein, putative glutathione peroxidase （GPX）, and translation initiation factor （elF-5A） were up-regulated in the resistant inbred line and were implicated in host defense response to C. lunata infection. It suggests that drought-inducible and oxidation stress-related proteins might directly contribute to maize resistance to C. lunata.@2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.Proteins differentially expressed from maize leaves in response to the infection by Curvularia lunata strain CX-3 were identified through a high-resolution two-dimensional gel electrophoresis (2-DE) method. Two inbred lines, 78599-1 and E28, were used, respectively, as resistant and susceptible lines to CX-3 infection. Proteins were extracted from the fourth leaves of sixor seven-leaf stage plants sampled at 24, 36, 48, 60, and 72 h after inoculation with CX-3. Twenty-seven differentially expressed protein spots resolved on the 2-DE gels were identified by MALDI-TOF MS/MS. The results showed that these proteins are associated with photosynthesis, respiration, oxidative and drought stress tolerance as well as signal transduction in maize. Among stress-related proteins, the 22 kDa drought-inducible protein, putative glutathione peroxidase (GPX), and translation initiation factor (eIF-5A) were up-regulated in the resistant inbred line and were implicated in host defense response to C. lunata infection. It suggests that drought-inducible and oxidation stress-related proteins might directly contribute to maize resistance to C. lunata.

关 键 词：Curvularia lunata MAIZE PROTEOMICS Two-dimensional electrophoresis （2-DE） 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]