花色苷抗炎抗肿瘤相关信号通路研究  

Study on signal pathways of cyanidin-3-glucoside in association with inhibiting inflammation and carcinogenesis by gene microarray

作　　者：王庆[1] 刘驰[1] 郭红辉[1] 胡艳[1] 夏敏[1] 侯孟君[1] 凌文华[1] 

机构地区：[1]中山大学公共卫生学院营养与食品卫生系,广州510080

出　　处：《中国公共卫生》2009年第7期800-801,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金重点项目(30730079);广东省自然科学基金重点项目(07117377)

摘　　要：目的用基因芯片观察花色苷矢车菊素-3-葡萄苷(C3G)作用人白血病单核细胞细胞株(THP-1)源性巨噬细胞前后相关信号通路上基因表达的差异。方法100μmol/L的C3G作用于THP-1源性巨噬细胞12h,用信号转导通路发现者基因芯片研究C3G对THP-1源性巨噬细胞基因表达谱的影响,并利用半定量逆转录-聚合酶链反应(RT-PCR)法对筛选出的部分基因进行验证。结果C3G作用后有7条信号通路中的15个基因发生改变,其中表达上调的基因有5个,包括肿瘤基因p53(TP53)、核因子κB抑制因子α(NFκBIA)、视黄醇结合蛋白基因2(RBP2)等;表达下调的基因有10个,包括FBJ鼠科骨肉瘤病毒癌基因同源物(V-fos)、诱导型一氧化氮合酶(NOS2A)、前列腺素内过氧化物合酶2(PTGS2)等,这些基因编码的宿主蛋白均为肿瘤免疫、炎症反应相关的信号转导通路中的关键点。半定量RT-PCR结果显示,经C3G干预后,NOS2A和PTGS2的表达水平下调了58%和54%。结论花色苷类植物化合物可能通过促有丝分裂、果蝇无翼基因同源物(WNT)、p53、磷脂酶C、核因子κB(NFκB)、Janus激酶/信号转导和转录活化因子(JAK/STAT)和维甲酸等信号转导通路发挥其抗炎、抗肿瘤功能。Objective To investigate the effect of cyanidin-3-glucoside （C3G）on gene expression relevant to signal pathway in THP-1 macrophage by DNA microarray for screening signal pathways of cyanidin-3-glucoside in associstion with inhibiting inflammation and carcingensis. Methods THP-1 macrophages were treated with 100 μmol/L C3G for 12 hr,then the expression levels of RNA transcipts were assessed using human signal transduction pathway finder microarray analysis, and some of the results were confirmed by semi-quantitative RT-PCR. Results The results showed that 15 genes in 7 signal transduction pathways were differentially regulated in the THP-1 macrophages at 12 hr post C3G-treatment. Five genes including TP53,NFKBIA, RBP2 were upregulated and 10 genes including FOS,NOS2A, PTGS2 were downregulated. These genes encode a host of proteins as the important targets in signal transducition pathways involved in inflammatiry response and carcinogensis. Conclusion Anthocyanins might inhibit the inflammatory response and carcinogensis via divergent signal pathways such as mitogenic pathway,Wnt pathway, p53 pathway, NFKB pathway, and JAK/STAT pathway.

关 键 词：花色苷 巨噬细胞 抗肿瘤 炎症 

分 类 号：R730.1[医药卫生—肿瘤]