脑脊液中兴奋性氨基酸对新生儿缺氧缺血性脑病的诊断意义  

The Change of Excitatory Amino Acids in Crebrospinal Fluid of Patients with Neonatal Hypoxic-Ischemic Encephalapothy and It's Effects

作　　者：俞生林[1] 张瑞宣[1] 朱玲悯 蒋滢[1] 黄美英[1] 

机构地区：[1]苏州医学院附属儿童医院,215003

出　　处：《苏州医学院学报》1998年第5期441-442,共2页Acta Academiae Medicinae Suzhou

摘　　要：应用丹酰氨一聚酰胺薄层层析法，测定34例新生儿缺氧缺血性脑病（HIE）及30例非HIE脑脊液（CSF）和血清中谷氨酸（GLU）、天冬氨酸（ASP）水平，CSF中两种氨基酸水平显著高于对照组（P＜0．001），且其含量与HIE病情严重程度呈正相关，（r＝0．72，P＜0．001），但与血清中GLU、ASP均无相关性，CSF中GLU、ASP含量测定可作为HIE诊断的一个指标。Concentrations of Glutamate (Glu) and Aspartate(Asp) in cerebrospinal fluid (CSF ) and serum in neonates sufferingfrom HIE(n = 34), control neonates (n = 30) without perinatal asphyxia and noncentral nervous system illness. were detected by dimethylaminonaphthalene － S － suffonyl chloride with thin layer chromatography (TIC ) and fluoromtn. The resulls showed that the Glu and Asp in CSF of HIE were significantly higher than that in the controls (P < 0. 001), and thelevels of G1u and Asp were posiliveIy correlated with the degree of HIE. Furthermore, in HIE group the Glu level was positively correlated with Asp in CSF (r = 0. 72, p < 0. 001); These was no Glu and relation between ASp in CSF and that inserum , (r = 0. 322, p > 0. 05,r = 0 .288, p > 0. 05 ). These results suggest that Glu and Asp neurotoxicity may play a criticalrole in the brain damage caused by neonate HIE and indicates that the determinatlon of Glu and Asp in neonates with HIEmay be helpful for the dignosis.

关 键 词：新生儿 缺氧缺血性脑病 脑脊液 氨基酸 

分 类 号：R722.120.4[医药卫生—儿科] R446.14[医药卫生—临床医学]