术前化疗对神经母细胞瘤细胞凋亡和增殖的影响作用  

The Effect of Preoperative Chemotherapy on Apoptosis and Proliferation of Neuroblastoma Cells

作　　者：高解春[1] 钱抱平[1] 金百祥[1] 

机构地区：[1]上海医科大学儿科医院外科

出　　处：《中华小儿外科杂志》1998年第4期193-194,共2页Chinese Journal of Pediatric Surgery

摘　　要：目的：探讨术前化疗对神经母细胞瘤细胞凋亡和肿瘤增殖的作用。方法：应用脱氧核糖核酸末端转移酶标记（ＴＵＮＥＬ）和增殖细胞核抗原（ＰＣＮＡ）免疫组化，观察术前化疗组６例和一期手术组１１例神经母细胞瘤标本的凋亡指数、增殖指数和分裂指数的差异。结果：术前化疗组凋亡指数（９．１７±４．８８）与一期手术组（１０．１１±５．１６）无显著差异，Ｐ＞０．０５；术前化疗组增殖指数（１８．００±９．５５）明显低于一期手术组（２９．００±１３．８０），术前化疗组的分裂指数（１．６７±１．３４）亦较一期手术组（３．６７±２．２４）有明显下降，Ｐ值均＜０．０５。结论：术前化疗对神经母细胞瘤的肿瘤细胞增殖有明显抑制作用。Objective: To evaluate the effect of preoperative chemotherapy on apoptosis and proliferation of neuroblastoma cells. Methods: Tumor cells were immunostained with terminal deoxytransferase mediated biotin DUTP nick end labeling (TUNEL) and proliferating cell nuclear antigen (PCNA). The indices of apoptosis, proliferation and mitosis of the chemotherapeutic group (receiving preoperative chemotherapy) were compared to those of the nonchemotherapeutic group (undergoing operation only). Results:Immunostaining with TUNEL revealed no significant difference between the chemotherapeutic and nonchemotherapeutic groups (9.17±4.88 vs 10.11±5.16, P>0.05). Immunostaining with PCNA showed that the proliferation index of the chemotherapeutic group was significantly lower than that of nonchemotherapeutic group (18.00±9.55 vs 29.00±13.80, P<0.05) and the mitosis index of the chemotherapeutic group was also lower than that of the nonchemotherapeutic group (1.67±1.34 vs 3.67±2.24, P<0.05). Conclusions: Preoperative chemotherapy is effective in suppressing tumour cells proliferation. Immunostaining with TUNEL and PCNA is useful in monitoring the apoptosis and mitosis of tumour cells.

关 键 词：神经母细胞瘤 术前化疗 细胞凋亡 细胞增殖 

分 类 号：R730.53[医药卫生—肿瘤] R730.264[医药卫生—临床医学]