Bcl-2基因增强表达对TNF诱导U937细胞凋亡的效应  

Effect of Overexpression of Bcl-2 on TNF-induced Apoptosis in U937 Cell

作　　者：王媛[1] 曾凡杰[2] 黄晓明[2] 胡向阳[2] 高翼之[2] 

机构地区：[1]解放军第81医院中心实验科 [2]南京铁道医学院科研所

出　　处：《南京铁道医学院学报》1998年第2期92-94,共3页Journal of Nanjing Railway Medical College

摘　　要：目的：探讨Ｂｃｌ－２基因增强表达对肿瘤坏死因子（ＴＮＦ）诱导的Ｕ９３７细胞凋亡的生物学效应。方法：用磷酸钙沉淀法将Ｂｃｌ－２基因转染到Ｕ９３７细胞，用Ｇ４１８筛选稳定转染子，用不同剂量ＴＮＦ处理后观察转染与未转染细胞的存活率。结果：用Ｂｃｌ－２稳定转染的Ｕ９３７细胞经ＴＮＦ处理，存活率明显高于未转染细胞。Objective:To study the biological function of overexpression of Bcl-2 in apoptosis induced by tumor necrosis factor(TNF) in U937 cell line.Methods:With calcium phosphate transfection,Bcl-2 expression vector was transfected into U937 cell.Positive cells were obtained through the G418 selection.The transfected and untransfected cells were treated by TNF,then,cell viability was assayed.Result:the survival level of Bcl-2 transfectant was far higher than the untransfected cells treated by TNF.Conclusion:Overexpression of Bcl-2 protect U937 cell from apoptosis induced by TNF.

关 键 词：肿瘤坏死因子 转染 细胞凋亡 BCL-2基因 

分 类 号：R730.21[医药卫生—肿瘤]