癌胚抗原真核表达质粒构建与鉴定  

Construction and Identification Of Eukaryotic Expression Plasmid Encoding Human Carcinoembryonic Antigen

作　　者：张彤[1] 张燕齐[1] 张光磊[1] 陈宗德[1] 阮丽荣[1] 杜献堂[1] 张绍祖[1] 

机构地区：[1]河南医科大学微生物学与免疫学教研室

出　　处：《河南肿瘤学杂志》1998年第3期161-163,共3页Henan Journal of Oncology

摘　　要：癌胚抗原（CEA）是一种重要的肿瘤相关抗原，同时也是针对某些肿瘤治疗的有效靶抗原。为了探讨运用表达CEA的重组质粒进行抗肿瘤治疗的可行性，本研究构建了两套表达CEA的真核重组质粒。方法：运用基因工程技术将编码人CEA的全长2．4kbcDNA分别插入到真核表达载体pcDNA3及VR1012中，并用多种限制性内切酶对重组质位进行酶切鉴定。结果：构建的重组质粒均含有24kbEA片段，且插入pcD－NA3及VR1012中的2．4kb片段的方向均为正向，将这两套重组质粒分别命名为pCEA－1及pCEA－2。结论：本研究结果为进一步研究表达CEA的重组质位的免疫效应打下了基础。Carcinoembryonic antigen (CEA)is an important tumor associated antigen and an effective target antigen for the therapy of several malignant malignant . In order to investigate the anti -- tumor effects of recombinant plasmids s expressing CEA, eukaryotic expression plasmids containing CEA cDNA were constructed. Methed: Genetic engineering techniques were applied to inserting a 2.4gb fragment encoding full -- length human CEA into two eukaryotic expression vectors pcDNA3, VR1012 respectively . The resultant recombinant plasmids were cleaved with appropriate endonucleases. Results: The insertion 2. 4kb DNA fragment was certified to be in the right orientation and the two recombinant plasmids were named pCEA -- 1, pCEA -- 2 respectively. Conclusions: The results paved the way for future research on the immunological effects of the two recombinant plasmids expressing CEA.

关 键 词：癌胚抗原 表达 质粒 构建 鉴定 

分 类 号：R730.3[医药卫生—肿瘤]