肿瘤转移抑制基因nm23-H1对不同宫颈癌细胞侵袭和增殖的作用  

Effects of tumor metastasis suppressor gene nm23-H1 on invasion and proliferation of cervical cancer cell lines

作　　者：杨琰[1] 卢实[1] 李敏芳[1] 王泽华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,湖北武汉430022

出　　处：《癌症》2009年第7期702-707,共6页Chinese Journal of Cancer

摘　　要：背景与目的:nm23-H1是一种多效性基因,其抑癌作用有一定的组织特异性。本研究目的在于探讨nm23-H1对不同宫颈癌细胞增殖和侵袭的作用。方法:将真核表达载体pcDNA3.1-nm23-H1转染入宫颈癌细胞,Transwell小室法检测转染前后细胞侵袭性改变。MTT法绘制生长曲线,比较细胞增殖能力。流式细胞仪检测细胞周期改变。结果:与亲本细胞Caski、SiHa和空载体转染细胞Caski-3.1、SiHa-3.1相比,pcDNA3.1-nm23-H1转染细胞Caski-N和SiHa-N的侵袭力和增殖受到明显抑制(P<0.05),G2/M期和S期细胞比例降低(P<0.05),而G0/G1期细胞比例明显增加(P<0.05)。nm23-H1对HeLa细胞增殖、侵袭及细胞周期均无明显影响(P>0.05)。结论:nm23-H1对不同宫颈癌细胞的增殖和侵袭等细胞表型可产生细胞特异性的抑制作用。Background and Objective： Previous studies have shown that nm23-H1 is a tumor metastasis suppressor gene. Nucleotide diphosphate kinase 1 （NDPK1） encoded by nm23-H1 is involved in cancer cellular differentiation, proliferation, apoptosis and metastasis. This study was to investigate the effects of nm23-H1 on proliferation and invasion of cervical cancer cells. Methods： The eukaryotic expression vector pcDNA3.1-nm23-H1 was transfected into cervical cancer cells. Cell invasion potential was determined by the Transwell assay. Cell proliferation was measured by MTT assay and changes in cell cycle distribution were analyzed by flow cytometry （FCM）. Results： Compared with parent cells （Caski and SiHa） and vector control cells （Caski-3.1 and SiHa-3.1）, the proliferation and invasion of pcDNA3.1-nm23-H1 transfected cells （SiHa-N and Caski-N） were apparently decreased （P〈0.05）, the proportions of GSM and S cells were obviously decreased while that of G0/G1 cells was increased （P〈0.05）. However, transfection of nm23-H1 gene had no influence on proliferation, cell cycle and invasion of HeLa cells （P〉0.05）. Conclusion. nm23-H1 gene could inhibit proliferation and invasion of cervical cancer cells in a cell-dependent manner.

关 键 词：rim23-H1 转染 宫颈肿瘤 增殖 侵袭 细胞周期 

分 类 号：R737.33[医药卫生—肿瘤]