层粘蛋白在人原发性肝细胞癌转移中的意义  

Significance of laminin in metastasis of hepatocellular carcinoma

作　　者：郑世曦[1,2] 陈汉琴[1,2] 杨军 阮幼冰[1,2] 武忠弼 

机构地区：[1]同济医科大学实验医学研究中心超微病理研究室 [2]武汉市职工医学院附属医院

出　　处：《华人消化杂志》1998年第6期507-509,共3页

基　　金：国家自然科学基金

摘　　要：目的了解层粘蛋白（ＬＮ）在原发性肝细胞癌（ＨＣＣ）转移中的作用和价值．方法应用放射免疫分析法（ＲＩＡ）、免疫组化和图象分析分别对１２例ＨＣＣ有转移者和１２例ＨＣＣ无转移者血清和癌组织中ＬＮ进行测定，并进行相关分析．结果ＨＣＣ有转移组患者血清中ＬＮ含量（μｇ／Ｌ）１７８±３２，显著高于ＨＣＣ无转移组１４０±２３（Ｐ＜００１）．ＨＣＣ有转移组患者组织中ＬＮ平均Ａ（ＯＤ值）为０１７±００６，显著低于ＨＣＣ无转移组０２６±００５（Ｐ＜００１）．组织中ＬＮ与血清ＬＮ含量成明显的负相关关系．免疫组化结果显示，ＬＮ不仅存在于间质中，还存在于癌细胞中；ＬＮ亦可排列成窦状，尤以ＨＣＣ转移者组织中为明显．结论ＬＮ在ＨＣＣ转移过程中具有双重作用。AIM To understand the significance of laminin (LN) in the metastatic potential of hepatocellular carcinoma (HCC). METHODS Radioimmunoassay (RIA) and immunohistochemistry as well as morphometry were used to determine the amount of LN in serum and tissues of patients with and without metastasis of HCC. Linear correlation was carried out between them. RESULTS Serum LN levels in HCC patients with metastasis were much higher than that without metastasis (μg/L, 178±32 vs 140±23, P <0 01). Morphometrically, the average A (OD) value of LN in tissues of the former were much lower than that of the latter (0 17±0 06 vs 0 26±0 05, P <0 01). There was a strong negative correlation between the amount of LN in the serum and that in the tissue. Immunohistochemically, LN lied not only in the stroma, but also in the cytoplasm of tumor cells. LN also maintained a sinusoidal like pattern, especially in tissues with metastasis. CONCLUSION LN plays double roles in the course of metastasis of HCC. Elevation of serum LN would be a simple and valuable screening test in predicting the metastatic potential of HCC.

关 键 词：病理 层粘连蛋白 肿瘤转移 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]