乳酸杆菌与丁酸梭菌联用治疗小鼠急性溃疡性结肠炎的分子机制的探索  

Investigating the molecule mechanism of combination of lactobacilli strain with clostridium butyricum strain on experimental ulcerative colitis

作　　者：刘凌云[1] 杨伟峰[2] 

机构地区：[1]湖南中医药大学第一附属医院药剂科,410007 [2]广州空军司令部医院内科

出　　处：《中国现代药物应用》2009年第13期8-10,共3页Chinese Journal of Modern Drug Application

摘　　要：目的观察联用乳酸杆菌和丁酸梭菌对小鼠急性溃疡性结肠炎的疗效并探讨其治疗机制。方法建立DSS诱导的小鼠急性UC模型,观察给予乳酸杆菌和丁酸梭菌治疗后,小鼠结肠黏膜的病理改变和Caspase-1和PCNA表达的变化。结果乳酸杆菌和丁酸梭菌可明显减轻小鼠结肠黏膜的损伤;可明显抑制Caspase-1的表达,同时,促进PCNA的表达,两菌合用组对Caspase-1和PCNA表达的影响作用最强。结论联用乳酸杆菌和丁酸梭菌对DSS诱导的UC有协同治疗作用,抑制结肠黏膜上皮细胞凋亡促进其增殖可能是它们发挥协同治疗作用的部分分子机制。Aim To investigate the molecule mechanism of combination of lactobacilli strain with clostridium butyricum strain on experimental ulcerative colitis. Methods To observed the effects of lactobacilli strain with clostridium butyricum strain , the mice ulcerative colitis was induced by Dextran sulfate Sodium （DSS） and tissue biochemical indexes and the expression of Caspase-1 and PCNA in the colonic mucosa were detected. Results Lactobacilli strain and clostridium butyricum strain significantly abbreviated the damage of colonic tissue and suppressed the expression of Caspase-1 and stimulated the expression of PCNA . moreover, there were the lightest histological damage and the lowest expression of Caspase-1 and the highest expression of PCNA in lactobacilli strain combined with clostridium butyricum strain. Conclusions Both lactobacilli strain and clostridium butyricum strain show therapeutic effect on DSS induced mice ulcerative colitis. The coeffects on ulcerative colitis were observed in combination of lactobacilli strain with clostridium butyricum strain. There are lower expression of Caspase-1 and higher expression PCNA in the combination group than that in the two strain group alone ,which may be the molecule mechanism of the coeffect on mice UC .

关 键 词：乳酸杆菌 丁酸梭菌 溃疡性结肠炎 

分 类 号：R574.62[医药卫生—消化系统] R996.1[医药卫生—内科学]