雷公藤内酯醇与人乳腺癌细胞大分子DNA作用模式的探讨  

Study about the Expression of ER and EGFR Receptors in Breast Carcinoma

作　　者：金晓明[1] 陆俐丽[2] 王小芹[1] 邓国仁 

机构地区：[1]福建省医学科学研究所生化研究室 [2]福建省肿瘤医院病理科 [3]北京市肿瘤防治研究所

出　　处：《福建医药杂志》1998年第3期97-99,161,共3页Fujian Medical Journal

摘　　要：采用药物与DNA作用的体外检测方法，以DNA嵌入剂表阿霉素（EDR）作对照，从分子药理水平研究雷公藤内酯醇（TL）对乳腺癌细胞DNA的作用模式。实验发现TL在120μg／ml浓度，37℃作用24h可与DNA模板结合，同EDR呈嵌合效应，但嵌入DNA尚不牢固，易发生解离；EDR在较低浓度（50μg／ml）作用30min即可显示明显的嵌合效应且是不可逆的。说明TL对乳腺癌细胞DNA的作用模式为嵌合逆行模型，嵌合能力远低于EDR，与DNA结合亦不可能是其抗癌主效应。The triptolide (TL)'s pattern of action with breast cancer cellular DNA at the molecular pharmacological level was investigated by using the epidoxorubicin (EDR),a DNA intercalative agent as a control and detective methed of drugs acted on DNA in vitro. The experiments discovered that the TL (120μg／ml) intercalated with the DNA at 37C for 24h was similar to the EDR in interactive pattern, but it intercalating into the DNA was not firm and dissociated easily. The EDR at lower concentration (50μg／ml) intercalated with the DNA for 30min and it was irreversible. The results demonstrated that the TL pattern of action with breast cancer cellular DNA belonged to an intercalation irreversible pattern, the intercalation of it with the DNA compared with the EDR differed greatly. The TL intercalating into DNA could not be also the main effect in antitumor.

关 键 词：雷公藤内酯醇 乳腺癌 细胞 DNA 

分 类 号：R737.9[医药卫生—肿瘤] R979.1[医药卫生—临床医学]