溶藻链霉菌SG-001发酵条件的优化及溶藻活性物质的理化性质  

Fermentation Conditions and Characteristics of Algal Active Substance by Streptomyces olivoreticuli SG-001

作　　者：孔赟[1] 缪礼鸿[1] 曾驰[1] 宋瑶[1] 王爽[1] 

机构地区：[1]武汉工业学院生物与制药工程系,武汉430023

出　　处：《湖北农业科学》2009年第6期1361-1364,共4页Hubei Agricultural Sciences

基　　金：国家"863"计划项目"新型生物饲料关键技术研究与新产品的开发"(2003AA241160)

摘　　要：从土壤中分离得到1株对铜绿微囊藻有明显抑制作用的橄榄网状链霉菌SG-001(Streptomycesolivoreticuli SG-001),对其发酵条件进行优化,并对其产生的抑藻活性物质进行了初步研究。结果表明,SG-001菌株发酵的最佳碳源和氮源分别为葡萄糖和氯化铵。SG-001菌株产生抑藻活性物质的最佳条件为葡萄糖10 g.L-1,氯化铵1.0 g.L-1,pH值7.0,培养温度28℃,发酵时间4 d。优化后铜绿微囊藻的叶绿素a去除率为67.23%,较之基础培养基提高了26.29%。SG-001菌株发酵液所产生的抑藻活性物质具有较好(121℃)的热稳定性,pH值8.0时活性最强。An algae-lysing actinomycetes SG-001 （Streptomvees olivoreticuli SG-001） was isolated from the soil, that showed very strong lysing effect on Microcystis aeruginosa.To optimize the fermentation medium of the strain and to study the characteristics of algal active substance produced by strain SG-001 ,the optium condition for active production of SG-001 was determined by orthogonal design,and the best conditions included： glucose 10 g·L^-1,NH4Cl 1.0 g·L^-1,pH value 7.0,temperature 28℃ and 4 days of fermentation time. The algal rate was 67.23%,which increased by 26.29% after the optimization. The algal active substance produced by SG-001 had better thermal stability at 121℃, strong activity at pH 8.0.

关 键 词：溶藻链霉菌 铜绿微囊藻 发酵条件 活性物质 

分 类 号：S154.39[农业科学—土壤学]