培养条件对枯草芽孢杆菌G8抗菌蛋白含量的影响及蛋白液对黄瓜菌核病的生防效果  

Effect of Fermentation Factors on Production of Antifungal Protein by Bacillus subtilis Strain G8 and the Biocontrol Efficacy of Antifungal Protein on Sclerotial Blight of Cucumber

作　　者：石怀兴[1] 尚玉珂[1] 季静[1] 慕卫[1] 刘峰[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018

出　　处：《农药学学报》2009年第2期244-249,共6页Chinese Journal of Pesticide Science

基　　金："十一五"国家科技支撑计划项目(2006BAD17B07)

摘　　要：为确定不同培养条件对枯草芽孢杆菌G8抗菌蛋白含量的影响,用硫酸铵沉淀法提取了不同pH,以及不同温度、装液量和培养时间下获得的发酵液中的抗菌粗蛋白,测定了其浓度和对黄瓜菌核病菌Sclerotinia sclerotiorum(Lib.)de Bary的抑制活性。结果表明,在Luria Bertani培养基条件下,适宜发酵产生抗菌蛋白的pH值、温度、装液量、培养时间和摇床转速分别为7.0、37℃、75mL/250mL(250mL容器中装75mL液体)、36h和180r/min。在此优化条件下,1L发酵液经硫酸铵沉淀后的蛋白量达48.4mg。对在上述条件下获得的G8抗菌粗蛋白进行了温室及田间试验。结果表明,浓度为310.5μg/mL的G8对黄瓜菌核病的保护效果和防治效果分别为76.14%和69.30%;G8抗菌粗蛋白与高岭土1∶2(V∶W)制成的膏剂对田间黄瓜茎部菌核病斑的治愈率为65.07%。To determine the factors of affecting production of antifungal protein from Bacillus subtilis strain G8, the fermentation broth cultured under different pH, temperature, inoculated size, incubation time was precipitated respectively by ammonium sulfate, and then the concentration of protein and the inhibition activity on Sclerotinia sclerotiorum （Lib.） de Bary were determined. The results showed that the antifungai protein displayed optimum activity with Luria Bertani Medium （pH 7.0 ） and shaking under 180 r/rain at presence of 30% （75 mL in 250 mL flask） broth content for 36 hours at 37℃. Under this optimal conditions, protein content achieves 48.4 mg in 1 L precipitated broth by ammonium sulfate. Using crude antifungal protein prepared under the above fermentation conditions at the concentration of 310.5μg/mL, the protective and curative efficacy on cucumber sclerotial blight was 76.14% and 69.30% ,respectively. The results of field trial indicated that the curative rate of a mixture of crude antifungal protein and kaolin [1： 2（ V ： W） ] on disease spot of cucumber shoot was 65.07%.

关 键 词：黄瓜菌核病 抗菌蛋白 发酵条件 生物防治 

分 类 号：S482.292[农业科学—农药学]