8-氯腺苷对生长因子诱导的视网膜色素上皮细胞增殖抑制作用的研究  

8 chloroadenosine inhibits the growth factor induced proliferation of retinal pigment epithelial cells in vitro

作　　者：杨萍[1] 张惠蓉[1] 

机构地区：[1]北京医科大学第三医院眼科

出　　处：《中华眼科杂志》1998年第4期270-272,共3页Chinese Journal of Ophthalmology

基　　金：博士学科点专项科研基金;国家自然科学基金

摘　　要：目的探讨８氯腺苷（８ｃｈｌｏｒｏａｄｅｎｏｓｉｎｅ，８ＣＬＡ）对生长因子诱导的视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍ，ＲＰＥ）细胞增殖的抑制作用。方法通过ＲＰＥ细胞培养，采用３ＨＴｄＲ掺入测定８ＣＬＡ对肿瘤坏死因子α（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ，ＴＮＦα）、白细胞介素１β（ｉｎｔｅｒｌｅｕｋｉｎ，ＩＬ１β）和碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）诱导的ＲＰＥ细胞ＤＮＡ合成的抑制作用。结果三种因子单独使用均可使ＲＰＥ细胞３ＨＴｄＲ掺入每分钟计数（ｃｏｕｎｔｐｅｒｍｉｎｕｔｅ，ＣＰＭ）值明显增高（Ｐ＜０．０５），８ＣＬＡ在其终浓度为２～１６μｍｏｌ／Ｌ时可使三种因子刺激的ＲＰＥ细胞３ＨＴｄＲＣＰＭ值明显降低（Ｐ＜０．０５）。在ＴＮＦα、ＩＬ１β、ｂＦＧＦ和８ＣＬＡ组，分别于１６、８、２μｍｏｌ／Ｌ时，ＣＰＭ值与基础值相近（Ｐ＞０．０５）。结论８ＣＬＡ可抑制生长因子诱导的ＲＰＥ细胞增殖。Objective To study the inhibitory effect of 8 chloroadenosine (8 Cl A) on growth factor induced proliferation of retinal pigment epithelial (RPE) cells in vitro.Methods Primary culture and subculture of bovine RPE cells were established in vitro first and then tumor necrosis factor α (TNF α), interleukin 1β (IL 1β), basic fibroblast growth factor (bFGF) plus different concentrations of 8 Cl A (2 16 μmol/L) were added to the cells of third passage respectively. DNA synthesis in RPE cells was measured with 3H TdR incorporation.Results Cells treated with TNF α, IL 1β, bFGF had a higher 3H TdR uptake than the basal. The increase was abolished by 8 Cl A in a dose dependent manner ( P <0.05). At TNF α, IL 1β, bFGF with 8 Cl A group, CPM was similar to basal at 16, 8,2 μmol/L respectively ( P >0.05).Conclusion 8 Cl A can inhibit the three growth factor induced proliferation of RPE cells.

关 键 词：8-氯腺苷 生长因子 视网膜病变 PRE 

分 类 号：R774.12[医药卫生—眼科]