随意引物PCR用于口腔链球菌基因分型的初步研究  

Polymorphisms generated by arbitrarily primed PCR in the oral streptococci: application to strain gene identification

作　　者：潘亚萍[1] 王红杨[1] 林长坤[1] 孙开来[1] 

机构地区：[1]中国医科大学

出　　处：《牙体牙髓牙周病学杂志》1998年第2期77-79,共3页Chinese Journal of Conservative Dentistry

基　　金：美国中华医学会CMB基金

摘　　要：目的：探讨口腔链球菌基因分型，比较细菌相互间基因差异。方法：设计２对１０碱基随意引物，提取变链球菌、血链球菌染色体ＤＮＡ，分别用一对引物进行ＰＣＲ扩增。结果：变链球菌、血链球菌扩增的ＤＮＡ基因片段有较大区别。设计不同序列引物，扩增的ＤＮＡ片段亦不同，可以分辨不同的基因位点。结论：随意引物ＰＣＲ检测方法稳定。Aim: To research oral streptococci strain gene identification and compare their difference. Methods: AP-PCR DNA polymorphisms technique was used to distinguish Streptococcus mutans and Streptococcus sanguis species by extracting chromosomal DNA and designing two 10bp arbitrarily primers. Results: There were large gene differences between Streptococcus mutans and Streptococcus sanguis, using different sequence primer the DNA polymorphisms was not same, but the main band of polymorphisms was alike. Conclusions: AP-PCR DNA polymorphisms method can rapidly identify bacterial species and strains, and be useful for the analysis for bacteria genomes.

关 键 词：随意引物 PCR 口腔科 链球菌 基因分型 

分 类 号：R780.2[医药卫生—口腔医学]