光固化型与化学固化型玻璃离子水门汀细胞毒性的比较  

Cytotoxicity of light-curing and self-curing glass ionomer cements in vitro

作　　者：郝建军[1,2] 李晨军[1,2] 史俊南[1,2] 于宏 王鑫源[1,2] 洪法廉[1,2] 

机构地区：[1]西安第四军医大学口腔医学院 [2]陕西省商业职工医院口腔科

出　　处：《牙体牙髓牙周病学杂志》1998年第2期104-106,共3页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金

摘　　要：目的：检测玻璃离子水门汀的细胞毒性，比较人牙周膜成纤维细胞、牙髓成纤维细胞和标准细胞株Ｌ９２９的敏感性。方法：用四唑盐（ＭＴＴ）显色法观察４种光固化型和化学固化型玻璃离子水门汀浸渍液的原液、１／２原液、１／４原液、１／８原液４个浓度对３种细胞的毒性作用。结果：光固化型玻璃离子水门汀Ｖｉｔｒｅｂｏｎｄ、化学固化的ＧＩ－１浸渍液的原液和１／２原液有较强的毒性，与对照组相比差别显著。其余两种和ＧＩ－１浸渍液的１／４原液和１／８原液呈较弱的体外细胞毒性。并且４种材料对３种细胞的毒性结果一致。结论：玻璃离子水门汀的细胞毒性与固化方式没有直接关系，原代培养的人牙周膜成纤维细胞。Aim: To assess the cytotoxicity of two light-curing and two self-curing glass ionomer cements (GIC) , and compare the cells' sensitivity to GICs among cultured human periodontal ligament fibroblasts,pulp fibroblasts and cell strain L929. Methods: The cytotoxicity of four GICs was examined using MTT colorimetric assay. Results: The cytotoxicity of Vitrebond, one of light-cured GICs, was significantly greater than that of the others. Cytotoxicity of each of four GICs to three kinds of cells was consistent. Conclusions: It seems that cytotoxicities of GICs are not directly connected with the curing modes of GICs.There is no obvious difference among three kinds of cells in the cytotoxicities of GICs.

关 键 词：玻璃离子水门汀 细胞毒性 MTT法 

分 类 号：R783.1[医药卫生—口腔医学]