化学酶法制备D-鸟氨酸盐酸盐  

Preparation of D-ornithine Hydrochloride by Chemoenzymatic Method

作　　者：刘莉 刘毅[2] 樊迪[2] 申伟松[2] 陶宁[2] 

机构地区：[1]江苏省徐州市环境保护科学研究所,江苏徐州221008 [2]江苏省脑病生物信息重点实验室徐州医学院,江苏徐州221004

出　　处：《安徽农业科学》2009年第20期9317-9319,共3页Journal of Anhui Agricultural Sciences

基　　金：江苏省高校自然科学基金资助项目(08KJD530004);徐州市科技计划项目(XM08C059);江苏省高校省级重点实验室开放课题(JSBL0803);江苏省高等学校大学生实践创新训练计划项目(2008)

摘　　要：[目的]探索制备D-鸟氨酸盐酸盐的新方法。[方法]采用L-鸟氨酸(L-Orn)为消旋反应原料,经消旋反应得到DL-Orn,然后利用蜂房哈夫尼菌AS1.1009中的赖氨酸脱羧酶进行DL-Orn酶法转化,可同时获得D-Orn和腐胺,并以此作为生物转化底物,考察了在水杨醛催化下,不同浓度NaOH水溶液对L-Orn消旋率和溶液中鸟氨酸总含量的影响。[结果]通过化学酶法制备的D-鸟氨酸盐酸盐,收率为46.1%。确定在回流条件下使用1.0 mol/L氢氧化钠水溶液,用0.10摩尔比的水杨醛催化L-鸟氨酸在3 h内可生成DL-鸟氨酸。对生物转化中赖氨酸脱羧酶性质的研究表明,添加5 mmol/L的Fe2+可将比酶活提高为6717 U。在此优化条件下,D-鸟氨酸可完全降解,最佳生物转化时间为16 h。[结论]该研究为制备D-鸟氨酸提供了一种新的方法。[ Objective ] The purpose was to explore the new method of preparing D-ornithine hydrochloride. [ Method ] With L-Ornithine （ L- Orn） as the raw material for racemization reaction, DL-Orn was obtained through the racemization reaction, and then DL-Orn and putrescine was produced by means of biotransformation of DL-ornithine with lysine decarboxylase in Hafnia alvei ASI. 1009 at same time, with which as the substrate of biotransformation, the effect of different concn, of NaOH water solution on DL-Orn rate and total amount of ornithine in the solution were investigated under salicylaldehyde catalysis. [ Result ] The D-ornithine hydrochloride prepared with chemoenzymatic method had yield of 46.1%. It was confirmed that in the water solution with 1.0 mol/L NaOH under return flow conditions, D-ornithine could be produced in the reaction process of racemizing L-ornithine by using 0.10 molar equivalent of salicylaldehyde within 3 h. The study on the character of lysine decarboxylase in biotransformation showed that adding 5 mmol/L Fe^2＋ ion could increase the specific activity up to 6 717 U. Under the optimization conditions, L-ornithine could be completely degraded by the decarboxylase, with the optimum time for biotransformation of 16 h. [ Conclusion] This study peocided a new method for preparing D-ornithine.

关 键 词：D-鸟氨酸 消旋 赖氨酸脱羧酶 底物特异性 

分 类 号：S188[农业科学—农业基础科学]