重组牛分支杆菌ESAT-6基因的表达与鉴定  

Expression and Identification of rESAT- 6 Gene From Bovine Mycobacterium

作　　者：李海涛[1] 刘艳环[1] 张广雷[1] 李庆超[1] 王志刚[1] 刘慧洋 苗利光[1] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109 [2]吉林市动物卫生监督所,吉林吉林132011

出　　处：《特产研究》2009年第2期5-7,共3页Special Wild Economic Animal and Plant Research

基　　金：国家科技支撑计划(2006BAD06B06)

摘　　要：在前期工作的基础上,构建了pGEM-TE-ESAT6重组载体,转化至大肠杆菌BL21(DE3)感受态细胞中,经诱导表达得到了20kD左右大小的重组蛋白,SDS-PAGE/Western Blot显示该重组蛋白是上清表达。为重组ESAT-6抗原的应用奠定了基础。Based on the previous work, ESAT-6 was cloned into pGEM-TE plasmid. After being transformed into ＋the BL21 （DE3）and inducted with IPTG, a protein about 20kD in size was expressed in the pGTE-ESAT6 system in a soluble form by SDS-PAGE/Western blot. It provides an experimental basis for the application of rESAT-6.

关 键 词：牛分支杆菌 ESAT-6基因 重组ESAT-6 

分 类 号：S852.618[农业科学—基础兽医学]