云南烟草上1种新的细菌性病害病原鉴定  

Identification of a new bacterial disease of tobacco in Yunnan Province

作　　者：余磊[1] 秦西云[2] 杜静[1] 汪安云[2] 赵莹莹[1] 商珊[1] 申敦晋[1] 李昊[1] 黄琼[1] 

机构地区：[1]云南农业大学植物保护学院,昆明650201 [2]云南省烟草科学研究所,玉溪653100

出　　处：《植物病理学报》2009年第3期243-248,共6页Acta Phytopathologica Sinica

基　　金：云南省烟草公司重大项目(06A06)

摘　　要：2006～2007年在云南文山州的西畴县和保山市的昌宁县发现1种由细菌侵染引起的烟草新病害。主要发生在苗期(漂浮育苗)和移栽期,症状开始为白色至暗绿色小斑点、湿腐和茎上出现褐色斑点,在KB培养基上产生荧光。根据致病性、菌体形态、培养性状、生理生化反应、Biolog测试和16S rDNA序列分析将该病原菌鉴定为铜绿假单胞菌(Pseudomonas aeruginosa)。尽管该病害曾在菲律宾发生,但在中国尚属首次报道。A new disease of tobacco seedlings was observed at different localities in Xichou County of Wen shan Prefecture and Changning County of Baoshan City, Yunnan Province from 2006 to 2007. The symptoms were common in the float beds and transplants with small, white and dark green lesions and brown lesion on stem, sometimes wet symptom was developed. A fluorescent bacterium was isolated from diseased tissues onto KB medium. These isolates were identified as Pseudomonas aeruginosa based on pathogenicity, morphology, physiological and biochemical characteristics. Biolog test analysis and sequence analysis of 16S rDNA showed the similarity to P. aeruginosa was 99%. The disease was first reported from Philippine. This is the first report of the disease from Yunnan, China.

关 键 词：致病性 BIOLOG 16S RDNA 铜绿假单胞菌 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]