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机构地区:[1]徐州医学院,江苏徐州221004
出 处:《山东医药》2009年第25期4-6,共3页Shandong Medical Journal
基 金:江苏省教育厅自然科学研究计划资助项目(04KJB310143)
摘 要:目的探讨生存素(Survivin)反义寡核苷酸(ASODN)诱导人胃癌细胞SGC7901凋亡的作用。方法设计合成特异性靶向Survivin的ASODN,将胃癌细胞株分为空白对照组(Sham组)、脂质体转染对照组(Lip组)、正义链转染对照组(Lip-SODN组)和ASODN转染组(Lip-ASODN组)。作用48 h后,Western blot法检测各组Survivin表达情况,流式细胞仪检测各组细胞凋亡率,免疫组化SP法检测细胞中PCNA表达情况。结果脂质体介导SurvivinASODN转染后的胃癌细胞Survivin蛋白表达明显下降;ASODN转染组细胞凋亡率明显高于各对照组(P均<0.05),各对照组间无统计学差异(P>0.05)。ASODN转染后胃癌细胞中PCNA表达水平明显降低。结论Sur-vivin ASODN转染胃癌细胞能下调Survivin蛋白表达,诱导胃癌细胞凋亡,抑制细胞增殖,具有明显的抗癌作用。Objective To investigate the apoptosis effects of Survivin antisense oligonucleotide (ASODN)on htunan gastric carcinoma cell SGC7901. Methods ASODN targeting Surviving was designed and constructed. Cultured cells were divided into control group, lipofectin group, oligonucleotide(ODN) group and ASODN group. After transfection for 48 h, Survivin protein expression was detected by Western blot; apoptotic rate (AR) was examined by flow cytometry; the expression of PCNA was detected in gastric cancer ceils by immunohistochemical staining. Results The expression of Survivin in SGC7901 cells decreased after transfected with Survivin ASODN. The AR of ASODN group was significantly higher than that of other groups test indicated that transfected ASODN downregnlated the expression levels of PCNA. Conclusions Expression of Survivin may decrease in gastric cancer cells after ASODN transfection. ASODN targeting Survivin can induce cell apoptosis, inhibit cell proliferation. Survivin ASODN is a prospective anti-cancer drug
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