戊四氮致痫大鼠学习记忆改变及海马CA_1和CA_3区神经颗粒素的表达变化  

Changes of Ng expression in hippocampal CA_1 and CA_3 of pentylenetetrazole-kindled rats with learning and memory impairment

作　　者：王海祥[1] 王洪新[1] 王维平[2] 王佩[3] 刘瑞春[2] 

机构地区：[1]天津市第四中心医院神经内科二病区,天津300140 [2]河北医科大学第二医院神经内科,河北石家庄050000 [3]保定市第一中心医院神经内科,河北保定071000

出　　处：《中风与神经疾病杂志》2009年第3期284-286,共3页Journal of Apoplexy and Nervous Diseases

基　　金：河北省自然科学基金(NO.C2007000852)

摘　　要：目的探讨戊四氮慢性点燃癫痫对大鼠学习记忆能力的影响及海马CA1、CA3区神经颗粒素(neurogranin,Ng)的表达变化。方法采用戊四氮(pentylenetetrazole,PTZ)腹腔注射慢性点燃癫痫(chronic epileptic,CEP)模型,用Morris水迷宫和Y迷宫对大鼠进行学习记忆能力检测,运用免疫组织化学方法测定大鼠海马CA1、CA3区Ng的表达变化。结果与对照组比较,慢性癫痫发作组大鼠在水迷宫中的逃避潜伏期延长(P<0.01),穿越平台次数减少(P<0.01),在Y迷宫中的错误反应次数增多(P<0.05)。在海马CA1区Ng的免疫反应强度减弱(P<0.05),CA3区两组比较差异无统计学意义。结论戊四氮慢性点燃癫痫大鼠学习记忆能力受损,海马CA1区Ng表达减少可能参与了这一过程。Objective To explore the effect of Pentylenetetrazole （PTZ）-kindled epilepsy on rats＇ learning and memory and the expression of neurogranin （Ng） in hippoeampal CA1 and CA3. Methods Use chronic model of epilepsy induced by PTZ intraperitoneal injection. The ability of learning and memory were assessed by the Morris water maze and Y maze. The expression of Ng in hippocampal CA1 and CA3 was determined by immunocytoehemical method respectively. Resuits The learning and memory ability of the epilepsy rats was impaired. Meanwhile, The immunological reaction of Ng for CEP group at CA1 was weaker than NC group,hut it made no difference at CA3. Conclusion The impairment of learning and memory ability of the epilepsy rats might be related with the changes of Ng in hippocampal CA1.

关 键 词：点燃 癫痫 学习记忆 海马 神经颗粒素 

分 类 号：R742.1[医药卫生—神经病学与精神病学]