脑缺血后尼膜同对AQP9 mRNA表达和血脑屏障通透性的影响  

Effect of Nimotop on the expression of AQP9 mRNA and the changes of BBB penetrability after cerebral ischemia in rats

作　　者：李燕华[1] 李吕力[1] 王铁建[1] 董艳玲[1] 李晓峰[1] 

机构地区：[1]广西壮族自治区人民医院神经内科,广西南宁530021

出　　处：《中风与神经疾病杂志》2009年第3期294-296,共3页Journal of Apoplexy and Nervous Diseases

基　　金：广西自然科学基金资助课题(桂科自0542063)

摘　　要：目的研究脑缺血后尼膜同对AQP9 mRNA表达和血脑屏障通透性的影响以及脑缺血后尼膜同对脑的保护作用。方法采用阻断大鼠大脑中动脉制作脑缺血大鼠模型,通过原位杂交和图像分析方法测定梗死区AQP9mRNA的表达水平,同时电镜进行对应部位的病理观察,并检测缺血脑组织中伊文思蓝外渗的量。结果脑缺血后,在尼膜同组和对照组缺血组织均出现缺血性病理变化、AQP9 mRNA表达上调、血脑屏障通透性增加,其变化趋势一致,随着脑缺血时间延长其改变加重。尼膜同组AQP9 mRNA的表达变化与生理盐水对照组,虽有差别,但差异无显著性意义(P>0.05);尼膜同组血脑屏障通透性及病理变化明显低于生理盐水对照组,其差异有显著性意义(P<0.05)。结论脑缺血后,尼膜同可能阻滞血脑屏障通透性的增加,起到脑保护作用,但不影响AQP9mR-NA的表达。Objective The aim of the study was to investigate the effect of Nimotop on the expression of AQP9 mRNA and the changes of BBB penetrability, as well as the effect of Nimotop on the brain after cerebral ischemia in rats. Methods The model of cerebral isehemia was made by occluding unilateral middle cerebral artery（MCA） of the rats with the suture method. The expression of AQP9 mRNA was assessed by in situ hybridization and imaging analysis. The pathological changes of the uhrastructure were observed under TEM. The BBB permeability of ischemic brain was determined by Evans Blue（EB） extravasation method. Results In Nimotop groups, the expression of AQP9 mRNA, the increasing of BBB permeability and the uhrastructure changes of the tissues were as same as those in control groups. The changes of two groups became remarkable after cerebral ischemia. Changes of AQP9 mRNA expression in Nimotop group were as same as those in control groups（ P 〉 0.05 ） ; BBB permeability and the uhrastructure changes were more slight in Nimotop group than in control group （ P 〈 0.05 ）. Conclusion Nimotop could decrease the permeability of BBB and brain damage after cerebral ischemia. However, Nimotop had no effect on the expression of AQP9 mRNA.

关 键 词：脑缺血 水通道蛋白-9 mRNA 血脑屏障 尼膜同 

分 类 号：R743[医药卫生—神经病学与精神病学]