白英提取物单独或与顺铂联合对宫颈癌Hela细胞的影响  

Effects of solanum lyratum thunb extract only or combined with cisplatin on growth of HeLa cells of cervical cancer

作　　者：黄宏思[1] 韦星[1] 韦鹏涯[1] 黄衍强[1] 

机构地区：[1]右江民族医学院免疫学教研室,广西百色533000

出　　处：《中国妇幼保健》2009年第20期2850-2852,共3页Maternal and Child Health Care of China

基　　金：广西青年科学基金项目(项目编号No:0640074)

摘　　要：目的:观察中药白英提取物(Solanum lyratum Thunb extract,STE)对体外培养宫颈癌HeLa细胞的作用,以及STE对HeLa细胞对顺铂(DDP)的敏感性的影响。方法:体外培养宫颈癌HeLa细胞分别用STE、DDP、DDP+STE处理细胞,MTT法检测细胞活性,流式细胞术检测细胞凋亡。结果:白英提取物及DDP对宫颈癌HeLa细胞的生长均表现出明显的抑制作用,低剂量STE可加强DDP对HeLa细胞的抑制作用;流式细胞术检测结果显示,白英提取物作用后HeLa细胞出现凋亡,且随着时间的延长,凋亡细胞的比例明显增加。结论:白英提取物对宫颈癌HeLa细胞有明显的抑制生长和促凋亡作用,同时能提高HeLa细胞对顺铂的敏感性。Objective： To investigate the effects of Solanum lyratum Thunb extract （STE） on HeLa cell line of cervical cancer cultured invitro and its effect on the sensitivity of HeLa cells to DDP, in order to find a purctraditional Chinese reagent to treat cervical cancer effectively and ultimately to optimize medication of cervical cancer. Methods： HeLa cells were treated with STE in different concentrations. The effects of STE on cell proliferation were tested by MTF assay. The effects of STE on cell morphology and apoptosis were studied by phase - contrast microscope and flowcytometry （FCM） respectively. Results： STE could obviously inhibit the proliferation of HeLa ceils in a time and dose dependent manner. It could induce apoptosis of HeLa cells. STE could also elevate the sensitivity of HeLa cells to DDP. Conclusion： STE can suppress proliferation of cervical cancer cells and induce apoptosis. It may be apure traditional Chinese reagent with non - side effects to prevent and treat cervical cancer effectively.

关 键 词：白英 宫颈癌 HELA细胞株 生长抑制 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]