新城疫病毒的制备纯化与初步鉴定  

Preparation and identify of Newcastle disease virus

作　　者：肖佩玉[1] 宋伟峰[1] 郑琼良[1] 

机构地区：[1]广州中山大学附属第三医院药剂科,广东广州510630

出　　处：《西部医学》2009年第7期1088-1090,共3页Medical Journal of West China

摘　　要：目的获得纯化的新城疫病毒,为下一步病毒的应用打下基础。方法利用传统鸡胚培养方法制备新城疫病毒(NDV),通过PEG-6000沉淀,差速离心和蔗糖密度梯度离心等方法提取纯化了新城疫病毒(NDV)。以血凝实验微量法测定其滴度和活性。用电镜及SDS-PAGE方法对纯化病毒进行鉴定。结果纯化病毒经磷钨酸复染后电镜观察,能看到典型病毒颗粒,SDS-PAGE电泳显示有较纯的蛋白条带。结论病毒纯化效果较好,为我们下一步病毒的功能研究及其应用打下了坚实的基础。Objective To acquire purified NDV virus to be foundation for next research. Methods Newcastle disease virus （NDV） was prepared in embryonic egg, NDV was purified by deposited of PEG-6000, speed centrifugation and sucrose--density gradient--centrifugation （SDGC）. The titer of NDV was detected with hemagglutination test. Purity NDV was identified with electron- microscope and SDS-PAGE. Results The typical virus particle was observe with electricmicroseope and NDV viruses were purified strap in SDS-PAGE. Conclusion NDV was successfully purified and may facilitate subsequent research of its properties.

关 键 词：新城疫病毒 纯化 SDS-PAGE 

分 类 号：R373[医药卫生—病原生物学] R73-36[医药卫生—基础医学]