云南食用百合病毒病的发生与检测  

Occurance and Detection of Viruses Infecting Yunnan Edible Lily

作　　者：王连春[1] 孔宝华[1] 赵丹[1] 陈海如[1] 李凡[1] 

机构地区：[1]云南农业大学云南省植物病理重点实验室,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2009年第4期518-521,共4页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省科技攻关项目(2003NG03);云南省自然科学基金重点项目(2004C0007Z)

摘　　要：通过田间调查、血清学技术、电镜技术、RT-PCR技术对侵染云南食用百合的主要病毒进行调查和研究,结果表明,食用百合病毒病的症状类型多为花叶,斑驳或者无症等类型。病毒种类主要是黄瓜花叶病毒(CMV),百合无症病毒(LSV)和百合斑驳病毒(LMoV)。其中通过ELISA检测,CMV的检出率达到53.3%,LSV的检出率达到46.7%;通过RT-PCR检测,CMV的检出率达到60%,LSV的检出率达到50%,LMoV的检出率达到30%。The main viruses infecting the Yunnan edible lily were studied by field investigation, ELASA, Electron microscopy and RT-PCR experiments. The result showed that the main symptoms types of these viruses were mostly mosaic, mottled symptomless and other symptoms. The main kinds of virus were Cucumber mosaic virus （CMV）, Lily symptomless virus （LSV） and Lily mottle virus （LMoV）. The results of ELISA showed that the positive rates of CMV and LSV were about 53.3% and 46.7%, respectively. The result of RT-PCR showed the positive rate of CMV, LSV and LMoV were 60% , 50% and 30% , repectively.

关 键 词：食用百合 黄瓜花叶病毒(CMV) 百合无症病毒(LSV) 百合斑驳病毒(LMoV) 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]