酶法提取杜仲多糖的工艺研究  

Study on Extraction Process of Eucommia ulmoides Oliv Polysaccharide

作　　者：刘晓河[1] 张海燕 梁惠花[1] 

机构地区：[1]河北北方学院医学院化学教研室,河北张家口075000 [2]河北省张家口市第一中学,河北张家口075000

出　　处：《河北北方学院学报（医学版）》2009年第3期24-26,共3页Journal of Hebei North University:Medical Edition

摘　　要：目的:确定杜仲中多糖的酶法提取工艺。方法:用正交试验的方法分别研究果胶酶、中性蛋白酶和纤维素酶对杜仲多糖提取率的影响。结果:果胶酶增加多糖提取率效果最为显著,其最佳提取条件是:酶加量0.10mg/L,酶处理温度50℃,酶处理时间3h。其次是中性蛋白酶,纤维素酶的效果最差。结论:果胶酶可明显增加杜仲多糖的提取率。Objective：To study the effect of extraction rate from Eucommia ulmoides oliv polysaeeharide by enzyme. Methods： It was investigated by orthogonal experiment design L9 （34 ） on the influence of the extraction rate of Eucomrnia ulmoides oliv polysaccharide, neutral protein enzyme and cellulose enzyme. Results：The effectiveness of enzyme was the best. The optimal condition of extraction was when enzyme was as much as 10% ,and 50℃ heat preservation up to 1h. The second was neutral protein enzyme,The cellulose enzyme was the last. Conclusion： The enzyme can increase the extraction rate of Eucommia ulmoides oliv polysaccharide.

关 键 词：杜仲 杜仲多糖 聚半乳糖醛酸酶 纤维素酶 正交试验 

分 类 号：R313[医药卫生—基础医学]