添加纤维二糖、淀粉水解液对纤维素酶制备的影响  

EFFECTS OF CELLUBIOSE AND STARCH HYDROLYSATE ON CELLULASE PRODUCTION ON STEAM EXPLODED CORN STRAW

作　　者：勇强[1] 陈艳萍[1] 余世袁[1] 

机构地区：[1]南京林业大学化学工程学院

出　　处：《南京林业大学学报（自然科学版）》1998年第2期53-56,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：中国科学院纤维素化学开放实验室资助

摘　　要：里氏木霉（ＴｒｉｃｈｄｅｒｍａｒｅｓｅｉＲｕｔＣ３０）以经蒸汽爆破预处理的玉米秆为底物制备纤维素酶，产酶ｐＨ值维持在５．０左右，底物浓度为３．７５％（玉米秆，干基）时，产酶３天，滤纸酶活力和纤维二糖酶活力达１．７６ＩＵ／ｍＬ和０．３８ＩＵ／ｍＬ，纤维二糖酶活力高，酶水解得率达８０．２％；在试验培养基中添加纤维二糖对纤维素酶的合成具有抑制作用，培养基中添加纤维二糖浓度在０．２５～１．５０ｇ／Ｌ时，滤纸酶活力和纤维二糖酶活力同时下降３０％～４０％；淀粉水解液对纤维毒酶合成的影响是由葡萄糖的抑制和复合糖的诱导共同作用的结果，在用本研究培养基制备纤维酶过程中添加淀粉水解液，从经济角度来说是不适宜的。Steam exploded corn straw has been examined as a candidate feedstock for both cellulase production and enzymatic hydrolysis. Batch cultivation of Trichderma reesei Rut c 30 on 3.75%(wt%) corn stalk yielded filter paper activity 1.76 IU/mL and high cellobiase activity (0.38 IU/mL)in three days. The yield of enzymatic hydrolysis on 10% steam exploded corn stalk was 80.2%. The study also investigated the effect of cellobiose and strach hydrolysate on enzyme production. It was found that cellobiose inhibited the enzyme production, the filter paper activity and cellobiose activity decreased by 30%～40% when cellobiose concentration was 0.25～1.50 g/L. The effect of starch hydrolysate on enzyme production was detemined by the inhibition of glucose and the inducement of olignosaccharides. It was found that the starch hydrolysate as a celluase inducer was uneconomical.

关 键 词：纤维素酶 纤维二糖 淀粉水解液 纤维素酶 制备 

分 类 号：Q556.03[生物学—生物化学]