超氧化物歧化酶和过氧化氢酶抗葡萄糖缺失诱导的神经元损伤作用研究  

Exogenous Superoxide Dismutase and Catalase Prevent Glucose Deficit-induced Damage of Neuron

作　　者：冯维杨[1] 李俞锦[1] 钱忠义[2] 

机构地区：[1]昆明医学院生物化学教研室,云南昆明650031 [2]昆明医学院病理教研室,云南昆明650031

出　　处：《昆明医学院学报》2009年第7期11-15,共5页Journal of Kunming Medical College

基　　金：云南省自然科学基金面上资助项目(2007CO70M)

摘　　要：目的探讨在葡萄糖缺失诱导的神经元损伤中,外源性超氧化物歧化酶和过氧化氢酶的作用.方法用不含D-葡萄糖的培养基诱导原代培养的神经细胞损伤.细胞凋亡、活细胞/死细胞百分比用Live/Dead染色法进行检测.细胞代谢活力测定采用alarmblue染色法检测.结果外源性超氧化物歧化酶和过氧化氢酶处理能降低葡萄糖缺失诱导的神经细胞凋亡率,增强神经元的代谢活力.结论外源性超氧化物歧化酶和过氧化氢酶对葡萄糖缺失介导的细胞损伤具有保护作用.deficit-induced Objective To investigate the effect of exogenous superoxide dismutase and catalase on glucose damage of neuron. Methods Neuron damage was induced by removal of D-glucose in neuro culture medium. The apoptosis of neuron was determined by Live/Dead staining. The metabolism viability of neuron was detected by alarmblue staining. Results Exogenous superoxide dismutase and catalase treatment resulted in increased the metabolism viability of neuron and decreased the rate of apoptosis in neuron. Conclusion Exogenous superoxide dismutase and catalase can prevent glucose deficit-induced damage of neuron.

关 键 词：超氧化物歧化酶 抗过氧化损伤 葡萄糖缺失 神经元损伤 

分 类 号：R742.89[医药卫生—神经病学与精神病学]