格林-巴利综合征相关空肠弯曲菌galE基因序列对比研究  

Comparison of galE gene of Campylobacter jejuni strains associated Guillain-Barre syndrome

作　　者：李鑫[1] 李震中[1] 白欣立[2] 刘慧[3] 赵子春[1] 刘卫卫[1] 方保峰[1] 王红[1] 郭医杰[1] 李春岩[1] 

机构地区：[1]河北医科大学第二医院神经内科,石家庄050000 [2]河北医科大学第二医院儿科,石家庄050000 [3]中国医学科学院北京协和医院神经内科

出　　处：《中华微生物学和免疫学杂志》2009年第6期555-558,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（30471919）;国家863计划（2006AA02A237）

摘　　要：目的测定3株格林-巴利综合征（Guillain-Barre syndrome，GBS）相关空肠弯曲菌的galE基因序列，并同GenBank中的空肠弯曲菌菌株相应序列进行比较，了解致GBS的序列特征并分析其遗传进化关系。方法选取分离自GBS患者粪便并经动物模型证实为致GBS的3株AMAN型空肠弯曲菌菌株进行培养并提取基因组DNA测序。将基因测序结果通过与NCTC11168菌株进行对照比较寻找galE基因突变位点并对galE基因片段进行遗传距离计算。结果3株致GBS空肠弯曲菌菌株的galE基因均由987个碱基构成。与NCTC11168的galE基因序列相比，此3株空肠弯曲菌菌株gale基因核苷酸序列有4个相同碱基突变并导致了4个对应的相同氨基酸突变。遗传距离计算，zhanxing株与qiaoyuntao株距离为1．5％，zhanxing株与lulei株距离为1．6％，qiaoyuntao株与lulei株距离为0．5％。结论GBS相关空肠弯曲菌中galE基因核苷酸序列的确存在相同变异且发生变异概率较非GBS相关空肠弯曲菌明显增大，遗传距离反映了此3株致GBS的空肠弯曲菌具有一定的区域特征。Objective To investigate the Campylobacterjejuni＇s risk factors which were associated with the development of Guillain-Barre syndrome （ GBS）, the galE gene of C. jejuni strains were sequenced and the sequencing results were compared with other C. jejuni strains. Methods Selecting three GBS-assoelated C. jejuni strains isolated from stools of GBS patients who had been diagnosed as AMAN pattern by clinical and electrophysiological test from Hebei province, China. After sequencing galE gene, the results were spliced and assembled into a complete sequence by the terminals overlapped each other. The sequences of galE gene were compared with the corresponding sequences in GenBank to find the mutation and constructed the phylogenetic tree. Results The variation frequency of gale sequences of GBS-associated C. jejuni were higher than that of non-GBS-associated C. jejuni. The phylogenetic analysis demonstrated that each of the three C. jejuni strains was separately genetically closed to three strains which sequences have published in GenBank. The alignment with the related sequence of NCTC11168 shows that there are 4 same mutations in the galE gene of the three C. jejuni strains. The phylogenic tree reflected the regional feature of C. jejuni. Conclusion The probability of sequence variation of galE of GBS-associated C. jejuni is significantly higher than non-GBS-associated C. jejuni strains, the relation between the variation and GBS-pathogenesis remains to be further confirmed. The mutations found in the three C. jejuni strains established the foundation for exploring the biologically characteristic of GBS-associated C. jejuni strains.

关 键 词：格林-巴利综合征 空肠弯曲菌 galE基因 序列分析 

分 类 号：R745.43[医药卫生—神经病学与精神病学] R373.31[医药卫生—临床医学]