塞来昔布联合顺铂对SGC-7901人胃癌细胞凋亡及Bcl-2表达的影响  

Effects of apoptosis and Bcl-2 expression by celecoxib with Cisplatin in SGC-7901 human gastric cancer cell line

作　　者：崔京远[1] 马红[1] 李梅[1] 杨洁[1] 张峻[1] 周东风[1] 

机构地区：[1]青岛大学附属青岛市立医院普外科,山东青岛266011

出　　处：《中国现代普通外科进展》2009年第6期466-468,共3页Chinese Journal of Current Advances in General Surgery

基　　金：青岛市卫生局科研基金资助项目(2004-wszd005)

摘　　要：目的:探讨选择性环氧化酶-2(COX-2)抑制剂塞来昔布联合顺铂对SGC-7901人胃癌细胞生长、凋亡及Bcl-2表达的影响。方法:终浓度为100、200及300μmol/L的塞来昔布作用于SGC-7901人胃癌细胞24h后加入顺铂(2.0μg/mL),采用MTT比色法测定细胞的生长抑制率,流式细胞仪检测细胞周期、细胞凋亡率及Bcl-2表达水平。结果:不同浓度的塞来昔布对SGC-7901人胃癌细胞均有抑制作用,抑制率与对照组相比,差异有统计学意义(P<0.05);流式细胞学检测到凋亡峰,细胞阻滞于G0/G1期。同时伴随Bcl-2表达水平下降,其抑制作用呈时间-剂量依赖性,且以上抑制作用与顺铂具有协同作用。结论:塞来昔布能促进顺铂诱导胃癌细胞凋亡,进而抑制胃癌细胞的增殖,这可能是COX-2抑制剂抗肿瘤的机制之一。Objective： To study the effects of celecoxib,a selective COX-2 inhibitor, with cisplatin on cell proliferation, Bcl-2 Expression and apoptosis in SGC-7901 human gastric cancer cell line and to explore its anti-neoplasm mechanism. Methods.. MTT assay was used to determine cell proliferation after incubation in different concentrations （[100,200,300μmol/L） of celecoxib with cisplatin （2.0 μg/mL）. Flow cytometry was adopted to examine apoptotic rate, cell cycle and Bcl-2 level. Results. Celecoxib significantly inhibited the growth of SGC-7901 human gastric cancer cells and elevated the inhibiting rate compared with control group （P〈0.05）. The apoptotic peak was detected with FCM and cells were blocked in G0/G1 stage . The inhibiting effects were in dose and time dependent manner, and were associated with decrease of Bcl-2 level： Conclusion： COX-2 inhibitor celecoxib can accelerate the apoptosis induced by cisplatin and inhibite the proliferation in SGC-7901 human gastric cancer Cell line. The results indicate one of the mechanisms of the anti-cancer effect of COX-2 inhibitor .

关 键 词：塞来昔布 顺铂 胃肿瘤 BCL-2 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]