肝细胞癌Tenascin蛋白和微血管密度检测及临床意义  

Detections of tenascin and microvascular density in hepatocellular carcinoma and their clinical significance

作　　者：张建平[1] 胡三元[2] 倪家连[1] 景洪标[3] 曹永成[3] 

机构地区：[1]济南军区总医院肝胆外科,山东济南250031 [2]山东大学齐鲁医院普外科,山东济南250012 [3]济南军区总医院病理科,山东济南250031

出　　处：《中国现代普通外科进展》2009年第6期473-476,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:研究细胞外基质Tenascin(TN)在肝细胞癌(hepatocellular carcinoma,HCC)中的表达,探讨TN对HCC肿瘤微血管生成、浸润、转移及预后的影响。方法:采用免疫组织化学方法检测63例HCC癌组织、癌旁组织及15例肝炎后肝硬化组织、10例正常肝组织中TN表达,同时检测HCC组织微血管密度(CD105-MVD),分析TN表达与HCC病理学特点及肿瘤微血管生成的关系。结果:HCC组织TN阳性率65.1%,癌旁组织69.8%,肝硬化组织20.0%,正常肝组织无阳性表达;HCC组织及癌旁组织TN表达阳性率及表达强度评分显著高于肝硬化组织及正常肝组织(χ2=27.67,P<0.01;F=12.82,P<0.01);HCC组织TN表达阳性率与癌旁组织比较差异无统计学意义(χ2=0.325,P>0.05);TN表达与HCC的组织学分级,病灶数量,有否包膜浸润、肿瘤转移及肿瘤微血管生成密切相关。结论:HCC组织TN的表达上调促进了肿瘤的新生血管形成,导致术后肿瘤的早期复发和转移;HCC组织TN检测可有效反映其恶性程度、浸润、转移及预后。Objective： To study the expression of tenascin in hepatocellular carcinoma （HCC） and to investigate the roles of tenascin expression in microvessel angiogenesis, invasion, metastasis and prognosis of HCC. Methods： Formalin fixed, paraffin wax embedded specimens from 63 HCC patients were stained with an anti-tenascin monoclonal antibody, and the measurements were also done for paracancerous specimens from the same patients and specimens from 15 patients with liver cirrhosis and 10 normal liver. The correlation was analyzed between expression of tenascin and clinicopathological features as well as microvessel angiogenesis. Results： Tenascin immunoreactivity was observed in 65,1% samples of HCC, 69.8% of paracarcinoma, 20.0% of liver cirrhosis and negative of normal liver samples, Positivity and intensity of tenascin expressions in samples of HCC and paracarcinoma were higher than those in samples of cirrhotic and normal liver. Tenascin expression was correlated with Edmondson-Steiner grades, tumour numbers, capsule invasion, tumour metastasis and microvessel angiogenesis. Conclusion： The up-regulation of tenascin expression may play an important role in invasion and metastasis of HCC.

关 键 词：癌 肝细胞 TENASCIN 微血管密度 

分 类 号：R735.7[医药卫生—肿瘤]