富血小板血浆与骨诱导活性材料复合物促种植体周骨缺损修复的实验研究  

Repairing of peri-implant bone defect with platelet-rich plasma and platelet-rich plasma/osteoinduction active material composite:An experimental study in dogs

作　　者：赵旺[1] 王秋旭[1] 刘维贤[1] 

机构地区：[1]中国医科大学附属盛京医院口腔科,辽宁沈阳110004

出　　处：《上海口腔医学》2009年第3期307-312,共6页Shanghai Journal of Stomatology

基　　金：沈阳市科学技术计划项目(1081269-9-00)~~

摘　　要：目的:观察富血小板血浆(platelet-rich plasma,PRP)、PRP复合骨诱导活性材料(osteoinduction active material,OAM)对种植体周围骨缺损的修复效果,探讨其作为种植体周围骨缺损修复材料的可行性。方法:Beagle犬4只,拔除双侧下颌第一、二、四前磨牙,3个月后植入种植体,制备种植体周围骨缺损并植入相应骨移植材料。A组植入PRP/OAM,B组植入PRP/磷酸三钙,对照组植入磷酸三钙。种植术后8、16周各处死2只动物,进行组织学观察,能谱分析种植体-新骨界面Ca2+含量,采用SPSS13.0软件包对数据进行单因素方差分析。结果:8周时,实验A组新骨与种植体形成区段性骨结合;实验B组种植体边缘可见新骨形成,但量较少;对照组种植体边缘为纤维性界面。16周时,实验A组可见哈佛系统,实验A、B组新骨与种植体形成骨整合;对照组为纤维性结合。能谱分析显示,8、16周时各组间钙含量百分比均存在显著差异。实验A组最高,实验B组其次,对照组最低。结论:PRP及PRP/OAM应用于种植体周围骨缺损中,可以促进骨缺损的修复,并形成理想的种植体-骨结合界面。PURPOSE： To investigate the effect of platelet-rich plasma （PRP） and osteoinduction active material （OAM）compounded with PRP in the repair of bone defects of peri-implant and evaluate the feasibility of PRP as a material for repair of peri-implant bone defects. METHODS： Four Beagle dogs underwent extraction of the mandibular first, second and fourth premolars to create edentulous regions. Three months later, titanium implants were inserted and bone defects around implants were created. The bone defects were repaired with PRP/OAM compounds in experimental group A, repaired with PRP/tricalcium phosphate in experimental group B, and repaired with tricalcium phosphate in the control group. The animals were sacrificed at the end of 8-week and 16-week, respectively. The specimens were observed with histological examination and energy disperse analysis of Ca^2＋. The data were analyzed with SPSS13.0 software package for one-way ANOVA. RESULTS： At 8-week, in experimental group A, some parts of implants were directly contacted with new bone. Some new bones formed in the experimental group B. There was fibrous connection between the bone and implant in the control group. At 16-week, there was mature Haversian system in the experimental group A. There was good osseointegration between the bone and implant in the experimental groups but only was fibrous connection in the control group. At 8-and 16-week, there was significant difference between there groups in the percentage of Ca^2＋ （P〈0.05）. CONCLUSIONS： PRP and PRP/OAM composite can effectively accelerate the regeneration of peri-implant bone defects and improve the osseointegration in the interface between the implant and bone.Supported by Research Fund Science and Technology Program of Shenyang City（Grant No.1081269-9-00）.

关 键 词：种植牙 骨缺损 富血小板血浆 骨诱导活性材料 骨整合 犬 

分 类 号：R783[医药卫生—口腔医学]