酸敏感离子通道在大鼠脑干斜方体核和旁巨细胞外侧核的表达及间歇性缺氧对其表达的影响  

Expression of Acid-sensing Ion Channels in Neurons of Trapezoid Body and Lateral Paragigantocellular Nuclei in Rat Brain,and Effects of Intermittent Hypoxia on Their Expression

作　　者：曹性玲[1] 陈祁[1] 周华[1] 唐玉红[1] 徐建国[1] 郑煜[1] 

机构地区：[1]四川大学华西基础医学与法医学院生理学教研室,成都610041

出　　处：《四川大学学报（医学版）》2009年第4期662-666,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30370530);教育部博士点基金(批准号20060610092)资助

摘　　要：目的探讨酸敏感离子通道(ASICs)的3种亚型(ASIC1b、ASIC2a和ASIC3)在大鼠脑干斜方体核(Tz)和旁巨细胞外侧核(LPGi)的表达以及在间歇性缺氧情况下三者表达的变化。方法制备间歇性缺氧大鼠模型,12d后抽血进行血气分析,采用常规免疫组化法(SABC法),观察正常大鼠及间歇性缺氧大鼠脑干Tz和LPGi神经元ASIC1b、ASIC2a和ASIC3的表达。结果正常大鼠脑干Tz和LPGi等神经核团,均可见到呈胞浆阳性的ASIC1b、ASIC2a和ASIC3表达的神经元。间歇性缺氧组大鼠,ASIC1b阳性神经元数密度(个/mm3)在Tz中减小(P<0.05),在LPGi中变化不明显(P>0.05),灰度值在Tz和LPGi中均无明显变化(P>0.05);ASIC2a阳性神经元数密度(个/mm3)在Tz和LPGi中改变均不明显(P>0.05),灰度值在Tz中增加(P<0.05),在LPGi中改变不明显(P>0.05);ASIC3阳性神经元数密度(个/mm3)在Tz中改变不明显(P>0.05),在LPGi中减小(P<0.05),灰度值在Tz和LPGi中改变均不明显(P>0.05)。结论正常大鼠脑干Tz和LPGi等呼吸相关神经核团神经元均表达ASIC1b、ASIC2a和ASIC3等ASICs;间歇性缺氧大鼠不同部位不同亚型ASICs表达的变化有所不同,提示不同部位不同亚型ASICs在呼吸调节中的作用可能有所不同。Objective To investigate the expression of three acid-sensing ion channel （ASIC） subtypes termed as ASIClb, ASIC2a, and ASIC3 in the neurons of trapezoid body and lateral paragigantocellular nucleus of rat brainstem, and the effects of intermittent hypoxia on their expression. Methods The intermittent hypoxic rat model was established, of which the blood gas analysis was tested after 12 days. The immunohistochemistry SABC method was performed to test the expression of ASIClb, ASIC2a, and ASIC3 in neurons of trapezoid body and lateral paragigantocellular nucleus in the control （O2） and intermittent hypoxic （IH） groups of rats. Results The ASIClb-, ASIC2a- and ASIC3-positive immunoreactive neurons all could be observed in the nucleus of trapezoid body and lateral paragigantocellular nucleus. The intermittent hypoxia group, the numerical density of ASIClbpositive immunoreactive neurons （cell/mm^3） decreased in the nucleus of trapezoid body （P〈0.05）, but did not significantly change in the lateral paragigantocellular nucleus （P〉0.05）, the grey level did not significantly change in both the nucleus of trapezoid body and lateral paragigantoeellular nucleus （P〉0.05）; the numerical density of ASIC2a-positive immunoreactive neurons （cell/mm^3） did not significantly change in the nucleus of trapezoid body and lateral paragigantocellular nucleus （P〉0.05）, the grey level in the nucleus of trapezoid body increased （P〈 0.05） while it did not significantly change in the lateral paragigantocellular nucleus （P〉0. 05）; the numerical density of ASIC3-positive immunoreactive neurons （eell/mm^3 ） decreased in the lateral paragigantocellular nucleus （P（0.05）, but it did not significantly change in the nucleus of trapezoid body （P〉0.05）, the grey level did not significantly change in the nucleus of trapezoid body and lateral paragigantocellular nucleus （P〉0.05）. Conclusion The ASIC1b, ASIC2a, and ASIC3 exist in the neurons of trapezoid body and lateral parag

关 键 词：呼吸调节 间歇性缺氧 酸敏感离子通道 大鼠 

分 类 号：R363[医药卫生—病理学]