抑制消减杂交筛选乳腺癌相关基因  

Screening Specific Expressed Genes of Breast Cancer by SSH

作　　者：王燕[1] 钟世军[1] 邵敏[1] 张青峰[1] 

机构地区：[1]遵义医学院珠海校区生化与细胞生物学教研室,广东珠海519041

出　　处：《中国民族民间医药》2009年第10期21-22,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:为了研究乳腺癌发生的分子遗传学机理,分离克隆模型鼠乳腺癌组织中特异表达的基因。方法:利用抑制消减杂交技术,结合反向cDNA斑点杂交试验,小鼠乳腺癌组织中分离出与正常乳腺组织差异表达的EST,应用生物信息学方法鉴定所得EST的小鼠同源基因,并分析其与乳腺癌发生的相关性。结果:获得20个在小鼠乳腺癌组织中高表达的,与已知的小鼠基因片段高度同源的基因,及2个与数据库中提交的假想蛋白基因高度同源的基因。结论:利用抑制消减杂交技术,可以高效高通量分析在乳腺癌中特异表达的基因,探讨乳腺癌发生的分子机制。Objective In order to study the molecular mechanism of breast cancer, to screen the genes differentially expressed in breast cancer. Methods Suppression subtractive hybridization （SSH ） combine reverse dot blotting was performed to isolate the differentially expressed genes between breast cancer and normal mammary tissues. A subtractive cDNA library of highly expressed genes in breast tumor tissue was established. Result About 22 differentially expressed genes were obtained. By sequencing and BLAST analysis we found 2 genes are new genes. Conclusion SSH is an effective method with high specificity to detect speeific expressed genes in breast cancer genesis and development.

关 键 词：抑制消减杂交技术 乳腺癌 基因 

分 类 号：R737.9[医药卫生—肿瘤]