Real-time Fluorescence PCR Method for Detection of Burkholderia glumae from Rice  

作　　者：FANG Yuan XU Li-hui TIAN Wen-xiao HUAI Yan YU Shan-hong LOU Miao-miao XIE Guan-lin 

机构地区：[1]State Key Laboratory of Rice Biology/Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China

出　　处：《Rice science》2009年第2期157-160,共4页水稻科学（英文版）

基　　金：supported by National Natural Science Foundation of China(Grant No.30671397 and No.30871655);the Public Beneficial Research Project of Agricultural Ministry,China(Grant No.nyhyzx07-056)

摘　　要：Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It＇s quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease. The present study combined the real-time PCR method with classical PCR to increase the detecting efficiency, and to develop an accurate, rapid and sensitive method to detect the pathogen in the seed quarantine for effective management of the disease. The results showed that all the tested strains of B. glumae produced about 139 bp specific fragments by the real-time PCR and the general PCR methods, while others showed negative PCR result. The bacteria could be detected at the concentrations of 1×10^4 CFU/mL by general PCR method and at the concentrations below 100 CFU/mL by real-time fluorescence PCR method. B. glumae could be detected when the inoculated and healthy seeds were mixed with a proportion of 1：100.Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It＇s quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease. The present study combined the real-time PCR method with classical PCR to increase the detecting efficiency, and to develop an accurate, rapid and sensitive method to detect the pathogen in the seed quarantine for effective management of the disease. The results showed that all the tested strains of B. glumae produced about 139 bp specific fragments by the real-time PCR and the general PCR methods, while others showed negative PCR result. The bacteria could be detected at the concentrations of 1×10^4 CFU/mL by general PCR method and at the concentrations below 100 CFU/mL by real-time fluorescence PCR method. B. glumae could be detected when the inoculated and healthy seeds were mixed with a proportion of 1：100.

关 键 词：Burkholderia glumae bacterial grain rot DETECTION real-time fluorescence polymerase chain reaction DCE 

分 类 号：TS207.4[轻工技术与工程—食品科学] TS207.3[轻工技术与工程—食品科学与工程]