凋亡诱导因子和caspase-3在结直肠腺瘤-癌序列中的表达及相关性  

Expression of AIF and caspase-3 in colorectal carcinoma, adenoma, and normal mucosa and its correlation

作　　者：关健婷[1] 王玲[1] 王永[1] 李园[1] 王蓉[1] 张文燕[2] 詹兰[1] 周总光[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室消化外科研究室,成都610041 [2]四川大学华西医院生物治疗国家重点实验室病理科研究室,成都610041

出　　处：《中华胃肠外科杂志》2009年第4期391-394,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：美国纽约中华医学CMB基金资助（CMB96636）

摘　　要：目的探讨非caspase依赖凋亡途径的凋亡诱导因子（AIF）与caspase依赖凋亡途径的caspase-3在结直肠腺瘤．癌序列中的表达变化及两种蛋白之间的相关性。方法采用免疫组织化学染色检测18例正常黏膜、84例结直肠腺瘤和72例结直肠癌中AIF及caspase-3的表达情况。结果结直肠腺瘤组织中AIF及caspase-3的阳性表达率均明显高于正常黏膜组织（P〈0．05）；腺瘤组织中AIF阳性表达率与腺癌之间的差异无统计学意义（P〉0．05），而caspase-3阳性表达率则明显高于腺癌组织（P〈0．05）；腺瘤组内绒毛状腺瘤AIF阳性表达率明显低于管状腺瘤（P〈0．05），而caspase-3阳性表达率在两种腺瘤之间的差异无统计学意义（P〉0．05）。相关分析显示，AIF表达与caspase-3表达无显著相关性（P〉0．05）。结论AIF代表的非caspase依赖凋亡途径异常发生在腺瘤向癌突变早期．而caspase依赖途径的凋亡失控可能是更重要的促进结直肠癌发生的因素之一。非caspase依赖的凋亡途径与caspase依赖的凋亡途径是肿瘤发生中两条相对独立的细胞凋亡途径。Objective To investigate the expression differences of apoptosis-inducing factor （AIF） and caspase-3 among colorectal carcinoma, adenoma and normal mucosa, and to identify the relationship between AIF and caspase-3 expression in colorectal adenoma-carcinoma sequence. Methods Formalin-fixed paraffin embedded colorectal tissues from 174 cases, including 84 adenomas, 72 carcinomas, and 18 normal mucosa, were examined for expression of AIF and caspase-3 by streptavidin-peroxidase（SP） immunohistochemistry. Results The positive rates of AIF and easpase-3 in colorectal adenoma were higher than those in normal mucosa （P〈0.05）. The positive rate of AIF in adenoma showed no significant difference compared to colorectal carcinoma （P〉0.05）. However, caspase-3 expression in adenomas was significantly higher than that in carcinoma （ P〈0.05 ）. The positive rate of AIF in tubular adenoma was significantly higher than that in villous adenoma （P〈0.05）, while the positive expression rate of caspase-3 in the two types of adenoma showed no significant difference （P〉0.05）. AIF expression had no prominent correlation with the caspase-3 expression （P〉0.05）. Conclusions The dysregulation of caspase-independent pathway of apoptosis may be an early event in the development of colorectal carcinogenesis, while the dysregulation of the caspase-dependent pathway of apoptosis may be one of contributing factors of colorectal carcinogenesis. The caspase-independent pathway of apoptosis and the caspase-dependent pathway of apoptosis are two relatively independent pathways in colorectal carcinogenesis.

关 键 词：结直肠肿瘤 腺瘤 凋亡诱导因子 半胱氨酸天冬氨酸蛋白酶3 

分 类 号：R735[医药卫生—肿瘤]