PRRSV缺失变异毒株JL/07/SW株的分离鉴定及序列分析  

Isolation,identification and sequence analysis of the strain JL/07/SW with deletion mutations of PRRSV

作　　者：李志杰[1] 丁壮[1] 孟轲音[2] 宣华[3] 王贵平[3] 高恩鹏[1] 丛彦龙[1] 母连志[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]军事医学科学院军事兽医研究所,吉林长春130062 [3]广东省农业科学院兽医研究所,广东广州510000

出　　处：《中国兽医学报》2009年第7期830-835,共6页Chinese Journal of Veterinary Science

基　　金：广东省农业攻关项目(2008A020100020)

摘　　要：分离并鉴定了1株猪繁殖与呼吸综合征病毒,经病毒生物学特性测定、血清学试验、病毒基因鉴定,确定为美洲型猪繁殖与呼吸综合征病毒,将其命名为JL/07/SW株。根据猪繁殖与呼吸综合征病毒VR-2332株及变异毒株的核苷酸序列,设计合成了针对NSP2变异序列、N基因以及GP5基因的引物,扩增出正确的序列,经测定的序列比对后,发现该毒株为美洲型变异毒株,NSP2上缺失了30个氨基酸,而GP5和M基因相对保守。One strain of PRRSV was isolated from tissue of piglets died of obvious respiratory syndrome and high fever. It was identified as a strain of American type PRRSV by serological test and gene sequencing. According to American type PRRSV VR-2332,we designed 3 pairs of primers to aim directly at N gene,GP5 gene and variation sequence of NSP2. The results of sequence analysis indicated a discontinuous deletion of 30 amino acids in nonstructural protein 2 （NSP2） . N and GP5 gene is conservative relatively.

关 键 词：猪繁殖与呼吸综合征病毒 分离 鉴定 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]