乳腺癌Akt的表达及其临床意义  

Expression of Akt and its clinical significance in breast carcinoma

作　　者：王秀玲[1] 益莉娜[1] 丁佩芬[1] 邹珏[1] 洪月琳[2] 郑唯强[3] 

机构地区：[1]上海市第七人民医院病理科,200137 [2]上海市第七人民医院外科,200137 [3]第二军医大学长海医院病理科,上海200433

出　　处：《临床与实验病理学杂志》2009年第3期257-259,263,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：上海浦东新区社会发展局基金资助项目(PW2006A-24)

摘　　要：目的本研究试图分析乳腺癌Akt的表达状况,并了解与其它有关生物学标记物的关系,为乳腺癌的治疗探索新的方法提供初步的理论基础。方法采用免疫组化EnVision两步法检测110例有随访资料的浸润性乳腺癌中pAkt和Akt的表达情况。结果乳腺癌pAkt和Akt2的阳性定位于肿瘤细胞的胞质或胞核。其pAkt和Akt2的阳性率分别为50%(55/110)和35.5%(39/110)。Akt2阳性的患者死亡率高于阴性患者,而pAkt阳性患者的淋巴结转移率及死亡率均高于阴性患者,差异具有统计学显著意义(P<0.01)。pAkt和Akt2均与患者的c-erbB-2的阳性表达率呈正相关。但二者与乳腺癌的癌基因p53、凋亡相关基因bcl-2、ER及PR的表达和肿瘤的组织学分级及患者年龄均无相关性。结论Akt的表达与c-erbB-2的过表达以及与患者较差的预后具有统计学意义的显著关联性,提示Akt的表达情况可能作为评估乳腺癌患者预后的参考指标之一;也表明尤其当患者出现对内分泌治疗抵抗时,摧毁PKB/Akt这一细胞内信号转导途径是否将更有利于乳腺癌的治疗值得进一步的研究。Purpose To analyse the Akt expression in breast carcinoma and to understand its relationship to biological markers so as to further explore the treatment for breast carcinoma. Methods pAkt and Akt expression was detected by using EnVision immunohisto- chemistry. Results Positive pAkt and Akt stains were detected in the nuclei or cytoplasm of the cancerous cells, pAkt expressed in 55/110（50% ） and Akt2 expressed in 39/110（35% ）. Higher positive pAkt and Akt2 expression was found in the group with lymph node metastasis and shorter survival, which showed statistical significance （P 〈 0. 01 ）. Expression of pAkt and Akt2 was positively related to c-erbB-2 expression, but not to p53, bcl-2, ER, PR, histologic grade and age. Conclusions Akt expression will probably be regarded as one of the markers to predict the prognosis of breast carcinoma, Whether to knock out the PKB/Akt signal will be benefit to the therapy of breast carcinoma needs further study.

关 键 词：乳腺肿瘤 AKT 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]