口腔癌相关成纤维细胞对Ⅰ型胶原蛋白降解作用的研究  

Investigation of the degradation of type I collagen co-cultured with oral carcinoma-associated fibroblasts

作　　者：刘英[1] 宋惠云[2,3] 李多[2] 李胜富[4] 周红梅[2] 

机构地区：[1]川北医学院附属医院口腔科,四川南充637000 [2]四川大学华西口腔医院黏膜科,四川成都610041 [3]郑州市口腔医院 [4]四川大学华西医院卫生部移植工程和移植免疫重点实验室,四川成都610041

出　　处：《临床口腔医学杂志》2009年第7期396-398,共3页Journal of Clinical Stomatology

基　　金：四川省教育厅青年基金(2005B019);教育部新世纪优秀人才支持计划(NCET07-0591)

摘　　要：目的:对比研究口腔癌相关成纤维细胞(CAFs)和正常成纤维细胞(NFs)对Ⅰ型胶原蛋白的降解能力。方法将Ⅰ型胶原蛋白凝胶与CAFs和NFs共同培养24h、48h、72h后,收集上清液,按照羟脯氨酸检测试剂盒说明测定羟脯氨酸含量。结果:CAFs上清超滤液中羟脯氨酸含量均值高于同时间段NFs组均值,并且作用24h、48h时两组降解作用均随时间的延长而增加。结论:CAFs降解Ⅰ型胶原蛋白降的能力强于NFs,提示CAFs与口腔鳞癌基质降解及肿瘤转移有关。Objective： To investigate the ability of carcinoma-associated fibroblasts （CAFs）and normal fibroblasts （NFs） to degradate the type Ⅰ collage. Method： CAFs and NFs were incubated on type Ⅰcollage for 24, 48,72 h. Equivalent amounts of conditioned medium were collected and assayed for hydroxyproline content. Result： CAFs were superior to NFs in hydroxyproline content at every times, the college degredation of each group was increasing with the time. Conclusion： CAFs were superior to NFs in degradation of I type collage,CAFs mighe act on degradation of extracellular matrix and metastasis of oral squamous cell carcinoma.

关 键 词：癌相关成纤维细胞 Ⅰ型胶原蛋白 降解 

分 类 号：R780.2[医药卫生—口腔医学]