癌相关成纤维细胞对雄激素非依赖性前列腺癌细胞生物学行为的影响  

Impact of Carcinoma-associated Fibroblasts on Biological Behavior of Androgen-independent Prostate Cancer Cells

作　　者：潘文海[1] 华兴[2] 

机构地区：[1]广州市红十字会医院泌尿外科,广州510220 [2]广州市红十字会医院病理科

出　　处：《临床泌尿外科杂志》2009年第7期549-552,共4页Journal of Clinical Urology

基　　金：2006年广东省社会发展领域科技计划项目(编号63101)

摘　　要：目的:探讨前列腺癌微环境中癌相关成纤维细胞(carcinorna-associated fibroblasts,CAFs)对非雄激素依赖性前列腺癌细胞株Du-145生物学行为的影响。方法:原代培养前列腺癌CAFs并制备CAFs条件培养基(CAFs-CM),通过增殖实验、平板集落形成实验、侵袭实验等体外实验,观察CAFs对前列腺癌细胞生物学行为的影响。结果:由从人新鲜前列腺癌组织中分离培养CAFs制备的CAFs-CM剂量依赖性地提高了Du-145细胞的增殖能力,与SFM相比,0.5 g/L的CAFs-CM明显增强了Du-145细胞的增殖能力,两者比较差异有统计学意义[(290.6±25.2)vs(88.4±12.1),P<0.05],其细胞增殖指数Ki-67明显提高(80%vs 20%);在CAFs-CM中培养的Du-145细胞与在DMEM中培养的细胞相比,其平板形成集落的能力明显提高,差异有统计学意义[(0.42±0.09)vs(0.23±0.03),P<0.05];在CAFs-CM中培养的前列腺癌Du-145细胞的变形迁徙破坏能力比在DMEM中培养的Du-145细胞明显增强[(162.12±9.13)vs(112.34±8.45),P<0.01]。结论:前列腺癌间质微环境中的CAFs促进了前列腺癌细胞的浸润与转移,是抗前列腺癌治疗药物设计的新靶点。Objective： To investigate the impact of cancer-associated fibroblasts（CAFs） in the microenvironment of prostate cancer on biological behavior of androgen-independent prostate cancer cell line Du-145. Methods： Prostate cancer CAFs was primary cultured and CAFs conditioned medium（CAFs-FM） was produced. Through the proliferation experiment, flat colony forming experiments and invasion assay to observe the impact of CAFs on the biological behavior of prostate cancer cells. Results：CAFs isolated from fresh human prostate cancer tissue and CAFs CM dose-dependent increased the Du 145 cell＇s proliferation capacity. Compared with SFM, 0.5 g/L CAFs- CM significantly enhanced the Du 145 cell＇s proliferation capacity, both there was a significant difference （290. 6 ± 25.2）vs（88.4± 12.1） （P〈0.05）, Du 145 cell proliferation index Ki-67 was eorrespongingly significantly im proved （80% vs 20%）. Compared with cultured in DMEM, fiat panel capacity of colony-forming and the move ment capacity of deformation were markedly improved, there were significant differences（0.42 ± 0. 09）vs（0. 23 0.03）（P〈0.05） and（162.12±9.13）vs（112.34±8.45）（P〈0.01） respectively. Conclusions：CAFs in the prostate cancer stromal microenvironment promote prostate cancer cell invasion and metastasis. CAFs is a new target for drug design in the course of the treatment of prostate cancer.

关 键 词：前列腺癌 癌相关成纤维细胞 浸润 转移 

分 类 号：R737.25[医药卫生—肿瘤]