四物汤配方颗粒对骨髓抑制小鼠性激素及其受体影响的实验研究  

Effect of Formula Granule of Siwu Drugs on Sex Hormone and Its Receptor in Bone Marrow Depressed Mice

作　　者：贾明昭[1] 夏青[1] 陈志伟[2] 

机构地区：[1]天津医学高等专科学校,300222 [2]齐齐哈尔医学院

出　　处：《天津医药》2009年第7期578-580,共3页Tianjin Medical Journal

基　　金：黑龙江省自然基金项目(项目编号:D2007-109);黑龙江省高校骨干教师资助项目(项目编号:1153G0)

摘　　要：目的:探讨四物汤配方颗粒对骨髓抑制小鼠性激素含量以及骨髓雌、雄激素受体蛋白表达的作用。方法:雄、雌性小鼠各90只,按小鼠性别分别分为正常组、模型组及四物汤配方颗粒低、中、高剂量组及阳性对照组共6组,每组15只,应用放射免疫法测定雌激素/雄激素(E/T)和免疫组化法测定雌激素受体(ER)和雄激素受体(AR)。结果:(1)雄鼠,雄激素方面,与模型组比较,给药组均呈现上升的趋势,差异有统计学意义(P<0.05);雌激素方面,与模型组比较,各给药组激素水平下降,差异有统计学意义(P<0.05)。(2)雌鼠,雄激素方面,与模型组比较,各给药组雄激素升高,差异无统计学意义(P>0.05);雌激素方面,与模型组比较,各给药组雌激素水平下降,差异有统计学意义(P<0.05)。各给药组间比较,具有剂量依赖性。结论:四物汤配方颗粒通过影响雄激素受体和恢复E/T比例平衡,进而发挥造血调控作用,修复骨髓抑制损伤。Objective： To investigate the effect of abstracted formula granule of Siwu drugs on the sex hormones and expressions of androgen receptor （AR）and estrogen receptor （ER） in bone marrow depressed mice. Methods： One hundred and eighty mice （90 male and 90 female） were randomly divided into 6 groups. The effects of the formula granule of Siwu on sex hormones and expressions of AR and ER were explored by SABC method in bone marrow of depressed mice. Results： For the male rats, the levels of androgen were increased in male of treatment groups compared with those of model groups （P 〈 0.05）, and the levels of estrogen were decreased in male of treatment groups compared with those of model groups （P 〈 0.05 ）. For the female rats, the levels of androgen were increased in female of treatment groups compared with those of model groups, but no significant differences （P〉0.05）, and the levels of estrogen were decreased in femal of treatment groups compared with those of model groups （P 〈 0.05）. There was a dose dependent manner between treatment groups. Conclusion： The results suggested that formula granule of Siwu drugs may play a role in the hematopoietic function to repair bone marrow depression by improving AR and the balance of estrogen / androgen in mice.

关 键 词：四物汤 骨髓疾病 小鼠 性腺甾类激素 受体 雌激素 受体 雄激素 放射免疫测定 免疫组织 化学 

分 类 号：R737.25[医药卫生—肿瘤] R730.53[医药卫生—临床医学]