泛耐药铜绿假单胞菌β-内酰胺酶基因分型及菌株亲缘性分析  

Genes of β-Lactamase in Pan-drug Resistant Pseudomonas aeruginosa and Phylogenetic Analysis

作　　者：糜祖煌[1] 秦玲[1] 金辉[2] 

机构地区：[1]无锡市克隆遗传技术研究所生物信息研究室,江苏无锡214026 [2]东南大学公共卫生学院流行病学与卫生统计学系,江苏南京210009

出　　处：《中华医院感染学杂志》2009年第13期1621-1623,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨泛耐药铜绿假单胞菌(PDRPA)β-内酰胺酶基因存在状况和菌株亲缘性。方法应用PCR检测21种β-内酰胺酶基因和整合子、转座子遗传标记。结果33株PDRPA blaTEM、blaOXA-10群、blaPER、blaGES、blaCARB、intⅠ1、merA基因阳性率分别为48.5%、45.5%、33.3%、21.2%、15.2%、78.8%、69.7%;聚类分析示存在克隆传播现象。结论PDRPA blaTEM、blaOXA-10群、blaPER、blaGES、blaCARB、intⅠ1、merA基因检出率高,PDRPA可存在克隆传播医院感染。OBJECTIVE To investigate the presence of β-lactamase genes in pan-drug resistant Pseudomonas aeruginosa （PDRPA）, and the redateness in PDRPA. METHODS The genes of 21 kinds of resistance were detected by polymerase chain reaction and phylogenetic analysis. RESULTS In 33 strains of PDRPA, the positive rates of blaTEM,blaOXA-10cluster, blaPER,blaGES,blaCARB, int Ⅰ 1 and merA were 48.5 %, 45.5%, 33.3%, 21.2%, 15.2% ,78. 8% and 69.7%, respectively. There were clone transmitted phenomena. CONCLUSIONS There are very high positive percentages of blaTEM, blaOXA-10cluster, blaPER, blaGES, blaCARB, int Ⅰ 1 and merA genes in PDRPA. The PDRPA can induce clone transmitted hospital infection.

关 键 词：泛耐药铜绿假单胞菌 Β-内酰胺酶 亲缘性分析 

分 类 号：R378.991[医药卫生—病原生物学]