通用引物PCR扩增创伤感染细菌16S-23S rDNA间区的研究  

PCR Amplification of 16S-23S rDNA Intergenic Spacer Regions of Bacteria in Trauma Infection by Universal Primers

作　　者：蔡晋[1] 夏季[2] 罗阳[1] 王珏[1] 许正林[3] 府伟灵[1] 

机构地区：[1]第三军医大学西南医院检验科,重庆400038 [2]大坪医院野战外科研究所检验科,重庆400042 [3]第三军医大学学员旅十队,重庆400038

出　　处：《中华医院感染学杂志》2009年第14期1776-1778,共3页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金(30571775);军队"十一五"课题(06G073);第三军医大学西南医院抗灾救灾专项课题(SWH2008008)

摘　　要：目的探讨通用引物PCR扩增多种创伤感染细菌16S-23S rDNA间区的可行性,为开展常见创伤感染细菌基因诊断做好准备。方法以细菌16S-23S rDNA间区为靶序列,在16S rDNA3′端与23S rDNA5′端保守区自行设计通用引物,筛选5种常见创伤感染细菌,提取其基因组DNA并进行PCR扩增,对扩增产物进行琼脂糖凝胶电泳和测序。结果5种细菌基因组DNAPCR扩增产物琼脂糖凝胶电泳图谱与预期一致,经测序比对后得到了证实。结论该通用引物可以实现对多种创伤感染细菌16S-23S rDNA间区进行PCR扩增,为下一步进行基因诊断打下了坚实的基础。OBJECTIVE To examine the feasibility of PCR amplification of 16S-23S rDNA intergenic spacer regions of bacteria in trauma infection by a pair of universal primers for gene diagnosis. METHODS The universal primers were designed at conserved regions of the 3＇ end of 16S rDNA and the 5＇ end of 23S rDNA. Bacterial genomic DNA from selected five commom bacteria in trauma infection were amplified by PCR. PCR products were examined using electrophoresis in agarose gel, and futher analyzed by sequencing. RESULTS The PCR products were similar to that we expected on the gel, which were confirmed by the results of sequencing and alignment. CONCLUSIONS Using the universal primers, 16S-23S rDNA intergenic spacer regions of bacteria in trauma infection could be amplified by PCR, which lays a solid foundation for gene diagnosis in farther studies.

关 键 词：通用引物 PCR 创伤感染细菌 16S-23S RDNA间区 

分 类 号：R446.5[医药卫生—诊断学]