HBV核心区基因突变的研究  

Genetic Mutations of HBV Core Region

作　　者：黄庆[1] 王贵宇[1] 黄君富[1] 潘虹[1] 李君[2] 府伟灵[1] 

机构地区：[1]第三军医大学西南医院检验科,重庆400038 [2]第三军医大学学员旅十队,重庆400038

出　　处：《中华医院感染学杂志》2009年第14期1793-1796,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金(30571775);军队"十一五"课题(06G073)

摘　　要：目的研究乙型肝炎病毒(HBV)核心区的突变模式。方法在核酸序列比对的基因上,设计靶向HBV各基因型核心区的通用引物,PCR扩增血清病毒DNA,经DNA测序和随后的序列分析,检测和分析核心区的突变模式。结果在34例可检测样本中,共有23例患者存在HBV核心区突变;其中,基本核心启动子区(BCP)的A1762T(50.0%)和G1764A(52.9%)是最常见的突变位点,并且,通常表现为双碱基突变;其次是核心(C)基因区的L60V氨基酸突变(17.6%);就各基因区的突变频率而言,BCP区、前核心(Pre-C)区和C基因区突变患者分别有18、6、10例,其中,BCP区通常表现为A1762T和G1764A双突变(94.7%),pre-C区以G1896A最为常见(83.3%),C基因区则主要是L60V的氨基酸单突变(50.0%)。结论HBV核心区最常见的突变形式是BCP区A1762T和G1764A的碱基双突变;由于HBV核心区基因具有复杂的突变模式,因此,研究HBV核心区基因突变模式对HBV感染的病情预测和预后判断具有重要的临床应用价值。OBJECTIVE To study the mutation profiles of hepatitis B virus （HBV） in the core regions. METHODS Based on the sequence alignment of all HBV genotypes, specific primers targeting all HBV genotypes were designed to amplify the core region of HBV followed by sequence analysis on the sequencing data available. RESULTS Among the 34 cases, 23 cases showed mutations in the core region. According to the mutation profiles, the most common mutations were the A1762T （50.0%） and G1764A （52.9%） in the basic core promoter （BCP） regions, and it always showed as double mutations. The L60V in core gene regions was the secondary common mutations （17.6%）. Among all patients, there were 18, 6 and 10 cases showed mutations in BCP, pre-core, and core gene regions, respectively. The most common mutations in BCP, pre-core, and core gene regions were the double mutations at A1762T and G1764A （94. 7%）, G1896A （83. 3%）, and L60V （50. 0%）, respectively. CONCLUSIONS The most common mutations in the core region of HBV are the double mutatidns at A1762T and G1764A. Analysis on the mutation profiles of HBV core regions might be helpful for the prognosis and prediction of HBV infections.

关 键 词：乙型肝炎病毒 DNA测序 基因突变 序列分析 

分 类 号：R446.5[医药卫生—诊断学]