苏太猪肌内脂肪沉积相关基因SRC-1的差异显示反转录PCR鉴定  

The identifition of SRC-1 gene by differential display RT-PCR related to intramuscular fat deposition in Sutai pigs

作　　者：薛春阳[1] 汪秀星[1] 王晓娜[1] 刘红林[1] 徐银学[1] 程占军[1] 陈杰[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《南京农业大学学报》2009年第3期126-129,共4页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金青年科学基金项目(30800780);国家863计划项目(2007AA10Z100;2006AA10Z136)

摘　　要：采取90头180日龄去势苏太猪的背最长肌组织测定肌内脂肪含量,从中选取肌内脂肪含量差异极显著(P<0.01)的最高和最低各6头组成RNA池,采用差异显示反转录PCR(DDRT-PCR)鉴定两组间差异表达的基因,结果共获得14条表达序列标签(expressed sequence tags),即EST序列,通过GenBank比对发现其中3条为已知序列。为进一步验证这3个已知基因与肌内脂肪之间的关系,从90个个体中选取肌内脂肪最高和最低各15个个体进行实时定量PCR检测。结果表明,细胞核受体共激活因子1基因(SRC-1)mRNA表达水平与肌内脂肪含量存在负相关关系(r=-0.38,P<0.05)。提示:SRC-1蛋白有可能参与了肌内脂肪沉积的调节。Longissimus dorsi （LD） muscle samples were collected from 90 castrated Sutai pigs aged 180 d and quantified intramus- cular fat （IMF） content. Two groups, each consisting of 6 individuals, of highest and lowest IMF content were selected to identi- fy the differential expressed genes between groups. Fourteen expressed sequence tags （EST） were gained and sequenced. The se- quences were compared with GenBank database. Three out of fourteen EST sequences were known genes. To further confirm the re- lation of these 3 genes with IMF content, 15 individuals with highest IMF content and 15 with lowest were exploded for mRNA ex- pression analysis by quantities real-time PCR. The mRNA levels showed significant inter-group difference for both storied receptor co-activator 1 gene （ SRC-1 ） （ P〈O. 05 ）. The result suggested that SRC-1 may be implicated in IMF deposition in pig LD muscle.

关 键 词：差异显示反转录PCR 肌内脂肪 实时荧光定量PCR 细胞核受体共激活因子1基因(SRC-1) 苏太猪 

分 类 号：S852.121[农业科学—基础兽医学]