壳聚糖游离膜在不同溶出介质中的降解性能研究  

Degradation property of chitosan free-film in different dissolution media

作　　者：曾敏[1] 谢兴亮[1] 杨明[1,2] 王森[1] 高亚男[1] 蒋巧梅[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《中草药》2009年第7期1050-1052,共3页Chinese Traditional and Herbal Drugs

基　　金：国家科技支撑计划项目(2006BA09B08-14);四川省中医药管理局专项资助项目(200615)

摘　　要：目的通过对比壳聚糖游离膜在不同酶液中的降解性能,筛选适宜于壳聚糖包衣结肠定位制剂体外的溶出介质。方法将壳聚糖制备成游离膜,以膜消化率、易碎性为评价指标,对其在复合果胶酶、进口果胶酶、胰酶、β-葡萄糖苷酶、大鼠结肠内容物等中的降解进行考察,并采用电镜扫描技术对变异性较大的膜表面性状进行观察,最终确定对壳聚糖降解最敏感酶液。结果大鼠结肠内容物、复合果胶酶、进口果胶酶对壳聚糖游离膜均有不同程度的降解,其中大鼠结肠内容物降解性能最强,游离膜在该介质中24h后成碎片,且膜表面最为粗糙;而游离膜对β-葡萄糖苷酶和胰酶不敏感。结论通过游离膜降解试验,明确了壳聚糖的敏感酶类,为结肠定位制剂体外溶出介质的选择提供了科学的依据。Objective Through comparing the degradation property of chitosan free-film in different dissolution media to screen dissolution media in vitro suitable for colon-specific delivery formulation by chi- tosan-coating. Methods Preparing the chitosan into free-film and taking the digestibility and fragmenta- tion as indices, the degradation of free-film in domestic compound pectinase, imported peetinase, pancreatin, β-gllucosidase, and rat colon contents were investigated. Aslo by electron microscopic observation to study film surface character on the great degrees of variation and finally determine the most sensitive en- zyme solution. Results Domestic compound pectinase, imported pectinase, and rat colon contents could degrade chitosan free-film in different degrees, where the rat colon contents was the most in which the free-film could be degraded into small pieces after 24 h and the surface was the most roughening, while the free film was not sensitive in panereatin and β-glucosidase. Conclusion By the degradation experiment of free-film, the sensitive enzyme on chitosan could be made clear by strong pertinency so as to provide the scientific basis for the selection of dissolution media in vitro on colon-specific delivery formulation.

关 键 词：壳聚糖 游离膜 酶解 结肠定位 

分 类 号：R286.02[医药卫生—中药学]