睾酮对亚急性衰老小鼠下丘脑的影响及其机制  

The effects of testosterone on the hypothalamus of the subacute aging mice and the mechanisms

作　　者：何天民[1] 吴赛珠[1] 阮云军[2] 邢晓雯[1] 刘新强[1] 罗才福[1] 赖文岩[1] 

机构地区：[1]广州南方医科大学南方医院心内科,广州510515 [2]广州军区总医院

出　　处：《中华老年心脑血管病杂志》2009年第7期539-541,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家重点基础研究发展计划(2007CB507400)

摘　　要：目的观察低刘量睾酮对小鼠下丘脑衰老的影响及其可能的调控机制。方法将24只健康雄性C57小鼠随机分为3组:D-半乳糖+睾酮治疗组(治疗组)、D-半乳糖组和正常对照组,每组8只。用药5个月后分离小鼠下丘脑组织,制备石蜡切片,测定小鼠下丘脑衰老相关性β-半乳糖苷酶(SA-β-Gal)染色情况,用免疫组织化学法检测p16^(INK4a)蛋白的表达水平。结果与正常对照组比较,D-半乳糖组小鼠体重降低(P<0.05),下丘脑SA-β-Gal染色阳性细胞率和p16^(INK4a)蛋白阳性细胞率明显升高(P<0.05);与D-半乳糖组比较,治疗组小鼠体重增加(P<0.05).下丘脑SA-β-Gal染色阳性细胞率和p16^(INK4a)蛋白阳性细胞率明显降低(P<0.05)。结论低剂量睾酮可能通过改变p16^(INK4a)的表达而发挥抗下丘脑细胞衰老的作用。Objective To observe the effect of low dose testosterone intervention on the expression of β-galactosidase and p16^INK4a in the hypothalamus of the aging mouse induced by D-galactose. Method Twenty-four C57 male mice were randomly divided into 3 groups：D-galactose＋testosterone group （n = 8） treated with testosterone 5 mg/kg per 2 weeks intramuscular injection and D-galactose 100 mg/kg per day subcutaneous injection in back;D-galactose group （n = 8） treated with D-galactose 100 mg/kg per day subcutaneous injection in back;normal control group （n = 8） treated with physiological saline per day subcutaneous injection in back. The activity of β-galactosidase and the expression of p16^INK4a in hypothalamus were determined after treatmemt for 5 months. Results Compared with the normal control group,in D-galactose group,the mice showed weight loss,hypothalamus SA-β-Gal staining positive cells and p16^INK4a protein positive cells were significantly increased. Compared with the D-galactose group, the mice in D-galactose＋testosterone treatment group showed weight gain, hypothalamus SA-β-Gal staining positive cells and p16^INK4a protein positive cells were significantly decreased. Conclusions Low dose testosterone could attenuate the aging of hypothalamus by reducing the expression of p16^INK4a.

关 键 词：睾酮 衰老 下丘脑 半乳糖苷酶类 

分 类 号：R363[医药卫生—病理学]