DSG型生物信息红外肝病治疗仪促进小鼠肝脏微循环的实验研究  

DSG biological information infrared liver disease therapy instrument improves liver microcirculation in mice

作　　者：田甜[1] 徐列明[2] 

机构地区：[1]上海中医药大学肝病研究所,201203 [2]上海中医药大学附属曙光医院肝硬化科上海中医药大学肝肾疾病病证教育部重点实验室上海市高校中医内科学E-研究院

出　　处：《中华临床感染病杂志》2009年第1期24-29,共6页Chinese Journal of Clinical Infectious Diseases

基　　金：基金项目：国家重点基础研究发展计划（973计划）部分资助项目（2006CB504800）;上海市重点学科建设资助项目（Y0302）;上海市教育委员会E-研究院建设计划项目部分资助项目（E03008）志谢 本文采用的活体显微视频技术得到深圳南山医院齐柯主任的帮助,特表谢意.

摘　　要：目的研究DSG型生物信息红外肝病治疗仪（BILT肝病治疗仪）对小鼠肝脏微循环的影响。方法将40只SPF小鼠造模后随机均分为未照射模型组、冷光源照射模型组、红外线照射模型组和BILT肝病治疗仪照射模型组，另取20只正常小鼠作为对照。每组分别以激光多普勒血流仪测定肝脏血流量并以显微活体视频技术观察肝脏微循环血流速度，同时进行肝脏病理学检查和血清生化学检查。结果BILT肝病治疗仪照射模型组肝脏平均血流灌注量与未照射模型组比较明显增加（P=0．004），而冷光源、红外线照射模型组与未照射模型组比均无明显增加血流量作用（P=0．713和0．465）。未照射正常组较未照射模型组小鼠肝脏微血管内红细胞平均流速快，差异有统计学意义（P=0．001）；BILT肝病治疗仪照射模型组肝脏微循环平均流速较未照射模型组、冷光源照射模型组和红外线照射模型组的流速也有所增加，差异有统计学意义（P=0．004、0．020和0．030）。BILT肝病治疗仪照射模型组AST活性较未照射模型组明显降低（P=0．027），该组羟脯氨酸（Hyp）平均含量低于未照射模型组，但差异无统计学意义（P=0．433）。结论BILT肝病治疗仪可改善肝纤维化小鼠肝脏的微循环并降低肝纤维化小鼠血清AST活性，改善肝脏病理状态。Objective To investigate the effect of DSG biologic information infrared liver disease therapy instrument （ BILT liver disease therapy instrument ） on liver microcirculations in mice. Methods Liver fibrosis was induced in 40 SPF mice which were randomized into 4 groups： without irradiation group, cold light irradiation group, infrared light irradiation group and BILT group （ 10 in each group）. Twenty healthy mice were taken as the control. Liver blood flow of each group was observed by laser-Doppler flow instrument, and blood flow rate of liver microcireulation in each group was observed by intravital microscopy. Pathological examination and serum biochemistry were also performed. Results In fibrosis mice, the liver blood flow in average of BILT group was higher than that of without irradiation group （ P = 0. 004 ） , while comparing with without irradiation group there were no significant differences in liver blood flows of the cold light irradiation group and infrared light irradiation group （P =0. 713 and 0.465 ）. Red blood cell velocity of mierovessels in average of the healthy group （ without irradiation ） was higher than that in the without irradiation group of fibrosis mice （P = 0. 001 ）, and it in the BILT group （fibrosis mice） was also higher than those in other groups of fibrosis mice （ P = 0.004, 0.020 and 0.030 ）. For fibrosis mice, the average AST in BILT group was lower than that in the without irradiation group （ P = 0. 027 ）, and the difference in hydroxyproline （Hyp） levels between the groups was not of statistical significance （P = 0. 433 ）. Conclusion BILT liver disease therapy instrument can improve the liver mierocir-culation, reduce AST level and improve liver pathological state in mouse with fibrosis.

关 键 词：纤维化 肝脏微循环 红外线 活体显微视频技术 

分 类 号：R575[医药卫生—消化系统]